Simultaneous Estimation of Ampicillin Sodium and Sulbactam Sodium in Injectable Dosage Form by High Performance Liquid Chromatography

Madhukar A. Badgujar and Kiran V. Mangaonkar*

Analytical Chemistry Research Laboratory SVKM’s Mithibai College, Vile-Parle (West), Mumbai - 400 056 (India).

Corresponding author: E-mail: bmbadgujar@yahoo.com

ABSTRACT:

A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Ampicillin sodium and Sulbactam sodium in injectable formulation.  The separation was achieved by Inertsil C18 column (250mm × 4.6mm i.d 5µm particle size) and Acetonitrile: Ammonium acetate buffer pH = 6.0 (17 : 83) as mobile phase at a flow rate of 1.0mL/min. The retention times of Ampicillin sodium and Sulbactam sodium were found to be 5.86mins & 4.33mins respectively. The proposed method was validated for linearity, accuracy and precision. The calibration curve was linear over the range of 50-150 µg/mL for Sulbactam sodium and 100 - 300 µg/mL for Ampicillin sodium. The proposed method can be applied successfully in routine analysis.

KEYWORDS:

Ampicillin Sodium (AMP); Sulbactam Sodium (SB); RP-HPLC; Validation

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Copy the following to cite this article: Badgujar M. A, Mangaonkar K. V. Simultaneous Estimation of Ampicillin Sodium and Sulbactam Sodium in Injectable Dosage Form by High Performance Liquid Chromatography. Orient J Chem 2011;27(4).

Copy the following to cite this URL: Badgujar M. A, Mangaonkar K. V. Simultaneous Estimation of Ampicillin Sodium and Sulbactam Sodium in Injectable Dosage Form by High Performance Liquid Chromatography. Orient J Chem 2011;27(4). Available from: http://www.orientjchem.org/?p=24248