ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Field Method for the Micro-Quantitative Determination of Tetracycline in Human Urine and Blood Serum

Subhash Chaudhary, Syed Kashif Ali and Y. P. Singh

Department of Chemistry, D.S. College, Aligarh - 202 001 (India).

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ABSTRACT:

This method described the determination of tetracycline in blood serum and it is based on the formation of an Eu(III)-tetracycline complex. Another spectrophotometric method2 utilizing the reaction of tetracycline with p-N, N-dimethylphenylenediamine and chloramines T was described. A sensitive liquid chromatographic method (10) has been adopted for the simultaneous determination of tetracycline, oxytetracycline and minocycline in serum. Tetracyclines were separated from other serum components by RPLC with buffered MeOH mobile phase. UV absorbance of the column effluent was monitered at 267 nm.

KEYWORDS:

Determination; tetracycline; urine and blood

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Chaudhary S, Ali S. K, Singh Y. P. Field Method for the Micro-Quantitative Determination of Tetracycline in Human Urine and Blood Serum. Orient J Chem 2010;26(1).


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Chaudhary S, Ali S. K, Singh Y. P. Field Method for the Micro-Quantitative Determination of Tetracycline in Human Urine and Blood Serum. Orient J Chem 2010;26(1). Available from: http://www.orientjchem.org/?p=23732



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