A Validated RP-HPLC Method for Estimation of Hepatitis B Surface Antigen (Hbsag) in Bulk Preparation

Vivek Shrivastava and U.K. Jain

Vaccine development cell, Bhopal Institute of Technology and Science-Pharmacy, Bhojpur road, Bhopal (India).

ABSTRACT:

A Simple Selective, rapid, precise and accurate reverse phase high pressure liquid chromatographic method has been developed for the estimation of Hepatitis B surface antigen (HBsAg) in pharmaceutical formulation. The method was carried out on a Gracesmart C18 5 micron (250 mm x 4.6mm i.d.) column with mobile phase consisting of 5 mmol potassium dihydrogen phosphate: Methanol (50: 50 v/v) and pH was unadjusted. The flow rate was kept 1.0 mL/min. Detection was carried out at 280 nm. The retention time of HBsAg was found to be 7.80 min. The developed method was validated for linearity, accuracy, precision (inter day and intra day), limit of detection and limit of quantitation.

KEYWORDS:

Hepatitis B; RP-HPLC; HBsAg; Surface antigen

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Copy the following to cite this article: Shrivastava V, Jain U. K. A Validated RP-HPLC Method for Estimation of Hepatitis B Surface Antigen (Hbsag) in Bulk Preparation. Orient J Chem 2009;25(3).

Copy the following to cite this URL: Shrivastava V, Jain U. K. A Validated RP-HPLC Method for Estimation of Hepatitis B Surface Antigen (Hbsag) in Bulk Preparation. Orient J Chem 2009;25(3). Available from: http://www.orientjchem.org/?p=23145