Abstract

Khaled.A.A.Alkadi¹, Aishah Adam¹, Muhammad Taha², Mizaton Hazizul Hasan², Syed Adnan Ali Shah^1,2*

DOI : http://dx.doi.org/10.13005/ojc/290304

Four known compounds, 5-Hydroxyflavone (1), 2’-Hydroxyflavanone (2), Paeonol (3) and bergenin (4),  were isolated from the methanolic extract of the stem bark of Garcinia malaccensis for the first time through combination of vacuum and radial chromatography techniques. The structures of the secondary metabolites (1-4) were elucidated on the basis of their spectroscopic evidence and comparison with the published data. All isolated compounds exhibited inhibition of platelet aggregation in human whole blood which induced by arachidonic acid (AA), adenosine diphosphate (ADP) and collagen. 2’-Hydroxyflavanone (2) showed inhibitory effect on platelet aggregation caused by two inducers with IC₅₀ 47.8± 2.1 AA and 147.2 ± 4.1 ADP.

Garcinia malaccensis;Platelet aggregation;Impedance Method;vacuum liquid chromatography (VLC);NMR spectroscopy

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