Validated reverse phase HPLC method for the determination of topotecan in pharmaceutical dosage forms

Murali Balaram Varanasi; Mushraff Ali Khan; Venkateswara Rao Jangala; Bulusu Bhanu

Article | Open Access

Validated reverse phase HPLC method for the determination of topotecan in pharmaceutical dosage forms

Murali Balaram Varanasi¹, Mushraff Ali Khan²,Venkateswara Rao Jangala²* and Bulusu Bhanu²

¹Department Of Pharmaceutical Analysis, Sultan-Ul-Uloom College Of Pharmacy, Banjara Hills,Hyderabad - 34 (India).

²Ther Dose Pharma Pvt. Ltd, IE, Kukatpally, Hyderabad - 500 072 (India).

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ABSTRACT:

A simple and gradient reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of topotecan in bulk drug samples and formulations. The method was validated for accuracy, precision, linearity, specificity, limit of detection and limit of quantitation. Topotecan was analyzed by using Zorbax SB – C18 (250 mm x 4.6 mm, 5

KEYWORDS:

Topotecan; high performance liquid chromatography; reverse phase liquid chromatography; validation