Separation of thaiamine mononitrate ascorbic acid calcium-D-pantothenate, Niacinamide. pyridoxine hydrochloride and riboflavin by using propyl hydroxy b-cyclodextrin as an ion-pairing reagent
1Arts, Commerce and Science College, Tukum, Chandrapur (India).
2Department of Chemistry, Saifia College of Science and Education, Bhopal - 462 001 (India).
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The present communication dipics the development of a reversed phase H.P.L C. for the simultaneous estimation of acidic and basic vitamins from solid dosage of polyvitamin and vitamin - B-complex tablets of therapeutic and prophylactic N.F.I. The seperation of vitamins was determined at 280nm. The proposed method is advantageous over reported method, the introduction of propyl hydroxy b-cyclodextrin as an ion-pairing reagent used and found to be profound effect over other ion-pairing reagent. The proposed methods are linear accurate and precise and applicable for daily quality control routine analysis
KEYWORDS:b-Cyclodextrin; Vitamin - B-complex; Ion-pairing reagent; separation of vitamins
