Determination of Tenofovir in Tablets by UV Spectrophotometric and Derivative Spectrophotometric Methods

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Two simple, rapid, sensitive and accurate UV- Spectrophotometric (method I) and first order derivative UV spectrophotomeric (method II) has been developed for estimation of tenofovir in bulk and tablets. In methanol – water (3:7), the lmax of Tenofovir was found to be 260 nm and the same spectrum was derivatized into first order derivative, using UV probe software of instrument (shimadzu 2450), at Dl = 2. The amplitude of the trough was recorded at 273 nm. In both the methods, linearity was observed in the concentration range of 5 – 40 μg/mL. The assay results were found to be in good agreement with label claim. The methods were validated statistically and recovery studies.

Tenofovir; UV-spectrophotometric method; first order derivative spectrophotometry