Spectrophotometric Methods for the Determination of Gatifloxacin

K. V. Siva Prasada Rao, C. Srinivasulu, P. Nagaraju, S. R. Sagineedu and G. Prabhakar

Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003 (India)

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ABSTRACT:

Three visible spectrophotometric methods have been described for the assay of gatifloxacin (GAT) either in bulk form or dosage forms. Methods A-C are based on the oxidation of GAT with an excess of oxidant [N-bromosuccinimide (NBS) in methods A and B or chloramine-T (CAT) in method C] in acidic medium. The unreacted oxidant is then estimated colorimetrically by using an oxidisable dye [Celistine blue (CB) in method A or Gallocyanine (GC) in method C] or by p-N-methyl aminophenol sulphate (PMAP)- sulphanilamide (SA) reagent in method B. Regression analysis of Beer's law plots showed good concentration range 2.0-10.0 mg/ml, 4.0-20.0 mg/ml and 1.0-5.0 mg/ml for methods A, B and C respectively and gives reproducible results.

KEYWORDS:

Spectrophotometry; NBS; CB; PMAP; SA; CAT; GC