Organic Synthesis of 3 β-Cholesterol Amine and its use in Affinity Chromatography

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3-β-cholesterol amine was synthesised from cholesterol by protection of location of double bond in cholesterol by Dromination at 5a, 6β position. The initial formation of bromine at 5a position was attributed to approach Br+ producing the cyclic brominium ion which was attacked at 6 carbon by continuation of bromination as colour started to change. Product was separated out as crystals and oxidised to synthesize 3β-cholestenone which was converted to 3β-cholesterol amine in presence of acidic sodium dichromate dihydrate. Oxime was reduced under the AcOH to get 3β-cholesterol amine.

3-β-cholesterol; Bromination; Crystals; AcOH