Abstract

Aayush G. Garg¹, Yash Raj Singh¹, Darshil B. Shah^1*, Dilip G. Maheshwari¹, Jignesh S. Shah²

DOI : http://dx.doi.org/10.13005/ojc/390329

RP-HPLC technique to estimate the Telmisartan and Rosuvastatin Calcium, employing an experimental design method using response surface methodology, was developed and validated. Experimental design was used to evaluate a multivariate optimisation of experimental conditions using flow rate, buffer pH, and % of acetonitrile in the mobile phase as three independent variables. The peak symmetry and retention time of the last eluting peak were optimized employing Derringer’s desirability function in which 1 ml/min flow rate, KH2PO4 buffer with pH 3.5 (altered with 1% ortho-phosphoric acid), and 65 % v/v of acetonitrile in the mobile phase in an isocratic proportion of acetonitrile: buffer (65:35, v/v) on a C18 column. Using response surface methodology, a RP-HPLC method was developed based on DoE that resulted in a better separation of peaks with a lower retention time of less than 9 min for eluted peaks. Response of linear was reported over the range of concentration of 20-100 μg/mL for Telmisartan and 5-25 μg/ mL for Rosuvastatin Calcium.

Chromatography; Design of Experiment; Pharmaceutical Analysis; Response Surface Methodology; Rosuvastatin Calcium; Telmisartan; Validation

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