Abstract

J. Dineshbabu¹, M. Helan Soundra Rani¹, B. Swathi¹, P. Srinivasan² and P. Rajiv^3*

DOI : http://dx.doi.org/10.13005/ojc/380220

Introduction: Diabetic Foot Ulcer (DFU) are the most common complication represent a central cause of morbidity among Diabetes Mellitus (DM) mostly type – ІІ, also reported amputation of foot region the impact of this complication cause mortality until treated. Biofilms are the sole responsible for over 90% of all chronic wounds in case of DFU. Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus sp, are prime pathogens causing biofilm, whereas higher prevalence occupied by Pseudomonas aeruginosa during chronic condition. Aim: The objective of the current investigation is to determine the value of methanolic fruit pulp extracts of Ficus racemosa and Aegle marmelos against isolated key biofilm former Pseudomonas aeruginosa and associated bacterial pathogens. Methodology: The phytochemical constituents for fruit pulp extracts of F. racemosa and A. marmelos were determined using FT-IR analysis. Minimum Inhibitory Concentration (MIC), Biofilm Inhibitory Concentration (BIC) and antibacterial activity were performed to find out the efficiency of the extract. Results: F. racemosa was shows the better antibiofilm activity than A. marmelos. Remarkably, Sub- MIC level showed increasing inhibitory activity as concentration increases (0.5mg/mL). Microscopic analysis showed dose dependant reduction in the biofilm architecture as compared to control. Conclusion: The extracts of fruit pulp of F. racemosa and A. marmelos show the anti-biofilm activity and which might be used as a substitute medicine in DFU. But it remains for the further analysis to elucidate the active binding molecule against biofilm former P. aeruginosa.

Biofilms; Diabetic Foot Ulcer; Ficus racemosa; Fruit pulp; MIC; Pseudomonas aeruginosa

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