ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Abstract

Ultrasound-Assisted Extraction of Phenolic, Flavonoid and Antioxidant Compounds from Dodonaea Viscose and Its Green Synthesis of Silver Nanoparticles by Aqueous Extract

Abdulaziz Ali Alomari

DOI : http://dx.doi.org/10.13005/ojc/360124


Abstract:

The Dodonaea viscose was commonly known plant in Al-mukhwah area (Al-baha city in Saudi Arabia) by exhibiting various therapeutic pharmacological properties owing to existence of divers polyphenols and flavonoids in its roots and seeds. Ultrasound-assisted extraction (UAE) was utilized to extract total phenolic and antioxidant compounds from Dodonaea viscose. The aim of this study is to inspect the solvent type effects, sonication time and dissipated power on the extraction of total phenolic compounds (TPC), total flavonoid compounds (TFC) and on the antioxidant capacity: 2,2′-azinobis-3-ethylbenzothiazoline-6 sulfonic acid (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The highest TPC was 176.63 mg. g-1 GAE of dry extract), whilst the total flavonoids equal 125.78 mg QE.g-1 of dry extract, the lower value IC50 of DPPH radical scavenging activity was 13.60 µg.g-1 of dry extract and under ABTS radical scavenging assay, IC50 was assessed as 3.58 µg.g-1 of dry extract. These results were observed for the sample extracted with pulsed ultrasound (40%) in Methanol at 15 min which indicated that the extracted compounds contribute to the activity of antioxidant. The natural products which are found in plants extracts, may be considered as a reducing agent as like a stabilizing agent in the formation of metal nanoparticles. Silver nanoparticles (Ag Nps) were synthesized by aqueous extract through a facile route. Characterizations of nanoparticles were evaluated by the use of UltraViolet-Visible spectroscopy (UV-Vis), Fourier Transform Infra-Red spectroscopy (FT-IR), and Transmission Electron Microscopy (TEM). Nanoparticles were found 30.20±19 (nm) by TEM analysis.

Keywords:

Dodonaea Viscose; Phenolic and Flavonoid Content; Antioxidant Activity; DPPH and ABTS; Ultrasound Mode, Solvent and Sonication Time Effect; Silver Nanoparticles.

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