Abstract

Gulzar Alama^1,2 and Arun K. Mishra¹

DOI : http://dx.doi.org/10.13005/ojc/360116

Manibhadra Yoga Polyherbal Formulation (MYPF) is an official classical polyherbal formulation, mentioned in Ayurvedic Formulary of India (AFI) and commonly indicated in skin’s diseases, abdomen’s problems, splenic disease, worm’s infestation and respiratory diseases. The two biomarker gallic acid and embelin are simultaneously estimated in in-house formulated MYPF by validated high performance thin layer chromatography (HPTLC) method. The developed HPTLC method was validated on the basis of precision, linearity, specificity, LOD, LOQ, accuracy and robustness. Aluminum backed TLC of silica gel 60F254 plate is use stationary phase while for mobile phase toluene: ethyl acetate: methanol: formic acid (5: 4: 0.5: 0.5 v/v) is used. LOD for gallic acid was found to be 75.24 ng/spot and for embelin was 81.60 ng/spot while LOQ for gallic acid was found 227.99 ng/spot and for embelin 247.28 ng/spot. Percentage recovery was found to be in range of 99.59-100.71 for gallic acid and 99.29-98.96 for embelin confirmed that the method was accurate. The content of gallic acid and embelin in in-house formulated MYPF were found to be 46.57 ± 0.814 & 16.15 ± 0.180 g/mg of formulation respectively which showed highest among all tested marketed formulations (MYPF-M1, MYPF-M2 & MYPF-M3). The proposed validated HPTLC method provides a new way for standardization and quantitative estimation of gallic acid and embelin in MYPF.

Embelin; Gallic Acid; HPTLC; MYPF; Standardization; Validation

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