Abstract

Nadezhda S. Komova, Anna N. Berlina , Anatoly V. Zherdev and Boris B. Dzantiev^*

DOI : http://dx.doi.org/10.13005/ojc/360103

Open-ring derivatives of beta-lactams do not inhibit bacterial growth but retain several biological activities. In this regard, methods of antibiotic control should be characterized by specificity to such derivatives. The objective of this study was to evaluate commercially available immunoreagents for the detection of beta-lactams from the point of view of their selectivity with respect to the open-ring derivatives of lactams. The enzyme-linked immunosorbent assays (ELISA) was used for this purpose. Ampicillin was hydrolyzed by acid and alkaline treatments, and opening of the lactam ring was confirmed spectrophotometrically. It was shown that either selective detection of only native ampicillin or integrated detection of both its states could be realized depending on the immunoreagents. The detection limit for native ampicillin for both assays was 10 ng/ml, whereas cross-reactivity to the derivatives with an open ring was <0.01% for the first case and 61.5–64.2% for the second case. The established variability is critical in interpreting the data of immunodetection and should be controlled for grounded evaluation of pharmaceuticals and foods.

Beta-Lactams; Destruction of Antibiotics; ELISA; Selectivity of Assays; Safety of Foods; Quality of Pharmaceuticals

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