Abstract

Ayoub Nadi^{1,2, 3}, Marouane Melloul⁴, Aicha Boukhriss¹, Elmostafa El-Fahime⁵, Damien Boyer³,Hassan Hannache⁶ and Said Gmouh*²

DOI : http://dx.doi.org/10.13005/ojc/350249

In the biotechnology field, nanoparticles with a strong magnetic moment can bring attractive and novel potentialities. They are detectable, manipulable, stimulable by a magnetic field and they could be applied as nano-tracers for medical imaging and nano-vectors for transporting therapeutic agents to a target. For our part, we applied Fe₃O₄ nanoparticles to immobilize bacteria of Moroccan strains in order to develop bacterial bioreactor. For this aim, we got through the synthesis and characterization of magnetite Fe₃O₄ nanoparticles by co-precipitation in basic medium. The obtained nanoparticles were encapsulated in silica by sol-gel process. The results of this step allowed us to use Fe₃O₄@SiO₂ nanoparticles to immobilize Bacillus licheniformis by adsorption and separate it magnetically. The principle of this system gives us the opportunity to develop a bacterial bioreactor for industrial applications.

Biotechnology; Cells immobilization; Iron Oxide Nanoparticles; Magnetic Separation; Sol-Gel

[ View HTML Full Text]