ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Abstract

Rosella (Hibiscus sabdariffa) Flowers as Alternative Indicators of Blue and Red Litmus

Siti Nuryanti1, Dwi Juli Puspitasari*2 and Supriadi1

DOI : http://dx.doi.org/10.13005/ojc/350163


Abstract:

The red and blue litmus indicators are an indispensable indicator for practicum in secondary school and higher education. The research has been done to make alternative indicator of litmus with rosella flower material. The procedures were rosella flowers were extracted, the filtrate was isolated by column chromatography, then the isolated compounds were analyzed using UV-Vis, FTIR, 1-HNMR. The alternative indicator of red litmus is made by acid-activated paper, then the paper was macerated into the isolated compound solution. Meanwhile, The alternative indicators of blue litmus were made by similar to the red litmus, but the paper was activated by base. The comparative indicators were blue and red litmus. The isolated compound which acts as an indicator is a cyanidin-3-glycoside compound. The color change of rosella indicator paper is the same as the comparison indicator.

Keywords:

Alternative Indicator; Blue Litmus; Red Litmus; Rosella Flowers

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