ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Abstract

Comparison of Iron Reduction Methods on the Determination of Antioxidants Content in Vegetables Sample

Yefrida, Hamzar Suyani, Hermansyah Aziz and Mai Efdi

DOI : http://dx.doi.org/10.13005/ojc/340525


Abstract:

Determination of antioxidant content in 14 vegetables has been done using Modified Phenanthroline (MPM), Phenanthroline (PM) and Ferric Reducing Antioxidant Power (FRAP) methods. The SDR values ​​obtained for the MPM and PM methods were (2.65 ± 1.26) % and (3.51 ± 0.88) %, while the recovery values ​​were (100.86 ± 4.45)% and (99.93 ± 3.50) %, respectively. The antioxidant content of vegetables obtained by MPM, PM and FRAP methods were (30.4 ± 1.46 - 515.9 ± 3.32), (25.9 ± 1.13 - 327.3 ± 2.57) and (21.4 ± 0.51 - 371.7 ± 1.10) μmol Fe / g DW, respectively. The antioxidant content obtained with the three methods showed no significant different. The correlation values ​​between MPM - PM, MPM - FRAP, and PM - FRAP methods are 0.9712, 0.9896 and 0.9611, respectively. These values indicate a very strong correlation among the three methods.

Keywords:

Antioxidant Content; FRAP Method; Iron Reduction Method; Modified Phenanthroline Method; Phenanthroline Method; Vegetables

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