Abstract

Kartika Dwi Kurniasari¹, Ade Arsianti^2,3, Yully Astika Nugrahayning Aziza¹, Baiq Kirana Dyahningrum Mandasari¹, Riathul Masita¹, Futihati Ruhama Zulfa¹, Micheylla Kusumaning Dewi¹,Cut Raisya Zahira Zagloel¹,  Norma Nur Azizah³ and Rista Putrianingsih²

DOI : http://dx.doi.org/10.13005/ojc/340308

In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, that is  n-hexane, ethylacetate, chloroform and ethanol. The extracts were analyzed by Phytochemical test and Thin Layer Chromatography (TLC). Subsequently, anticancer activity of n-hexane, ethyl acetate, chloroform and ethanol extracts of Gracilaria verrucosa were evaluated against colorectal HCT-116 cells by MTT cell proliferation assay. Based on Phytochemical analysis, the extracts of Gracilaria verrucosa containing secondary metabolite of saponin, while TLC analysis indicated that the extracts were composed by five chemical compounds. Among four concentrated extracts of Gracilariaverrucosa, ethanolic extract showed the strongest anticancer activity against colorectal HCT-116 cells with IC₅₀ of  43.9 μg/mL.Ethanolic extract of seaweed Gracilaria verrucosa is potential to be further developed as a promising anti-colorectal cancer agents.

Anticancer Activity; Colorectal HCT-116 Cells; Gracillaria Verrucosa; Phytochemistry

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