Abstract
Formulation of Liposome using Sappan Wood (Caesalpinia Sappan L.) Ethyl Acetate Fraction as an Activate Compound
Febriyenti, Deni Noviza, Henny Lucida, Netty Suharti and Putri Bhana Pinto
DOI : http://dx.doi.org/10.13005/ojc/340355
Abstract:
The purpose of this study is to determine the characteristics of liposome containing Sappan wood ethyl acetate fraction (SWEAF) as an active compound. Liposome was prepared by using two methods: Thin Layer Hydration (TLH) and Reversed Phase Evaporation (REV) methods. Liposome composition was 1 part of the SWEAF and 6 parts of the liposome component (phosphatidyl choline and cholesterol with an equally large molar ratio). Both methods produce red-colored liposome dispersion. When examined with transmission electrone microscope, both methods yielded a spherical and unilamellar liposome. The TLH method produces liposome with a particle size of 2,304.5 ± 528.9 nm. The REV method yields a liposome with a particle size of 2,643.5 ± 350.8 nm. The polydispersity index shows the distribution of liposome size. The TLH method produces liposome with a polydispersity index of 1,490 ± 0.284. The REV method produces liposome with a polydispersity index of 1.987 ± 0.901.
Keywords:Caesalpinia sappan L.; liposome; Thin Layer Hydration Method; Reversed Phase Evaporation Method
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