Abstract

Eman Ramdan Elsharkawy^1,2

DOI : http://dx.doi.org/10.13005/ojc/340331

Nowadays there is world concentration with synthesis of nanoparticles especially silver and gold nanoparticles, by green method for use in many application, exporting antimicrobial effect, Artemisia monosperma is medicinal plant have potent, anticancer, antispasmdial activity, The study aim to synthesis AgNPs from aqueous plant extract by green method to avoid the chemical toxicity biosynthesis and assay the antibacterial activity of synthesis AgNPs. The nanoparticles produced were further characterized using, UV, IR, and  Transmission Electron Microscopy (TEM). TEM analysis confirm the synthesis of silver nanoparticles, (AgNPs) which have spherical  shape and an average size of 17 nm. Antibacterial assay of capped AgNPs against Gram-positive and Gram-negative bacteria,  revealed that AgNPs had good  antibacterial activity than aqueous extract under condition prepared, each naonparticle and aqueous extract haven't activity toward Candida albicans. GC-MS of plant extract investigate  many important compounds, flavonoid (Kumatakenin), alkaloid (Aspidospermidin-17-ol) and other organic compounds (methyl-jasmonate and verbenol). These results showed that AgNPs can efficacy used in biotechnological and biomedical applications.

Gram-Positive; Gram-Negative Bacteria; Silver Nanoparticles; Artemisia Monosperma

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