Abstract

B. Sidda Reddy^{1, 2}, Y. Sudhakar², Y. Subba Rao³, P. Reddyprasad¹ and N. Y. Sreedhar¹

DOI : http://dx.doi.org/10.13005/ojc/330539

A modest liquid chromatography/tandem mass spectrometric method was developed and validated to determine three tetracycline antibiotics and their epimers tetracycline, oxytetracycline, chlortetracycline, 4-epi tetracycline, 4-epi oxytetracycline, 4-epi chlortetracycline in shrimp samples respectively. The procedure was involved the extraction of tetracyclines and its epimers from homogenized sample with EDTA-McIlvaine buffer followed by solid phase extraction clean up with Oasis HLB cartridge.  The analysis was carried out using Inertsil ODS-3V, 5 micron, 150×4.6 mm column and mobile phase consists of 5 mM Oxalic acid in water (A) and 0.1% Formic acid in methanol (B) in gradient mode at a flow rate of 0.8 mL/min. The final extracts were analyzed by the sensitive and selective LC/ESI/MS/MS operating in positive ion multiple reaction monitoring (MRM) mode.

Tectracylines; Antibiotics; Shrimp; Liquid Chromatography; Tandem Mass spectrometry

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