Abstract

Suryadevara Vidyadhara^1*, Yarraguntla Srinivasa Rao², Anne Ramu¹, Reddyvalam Lankapalli Sasidhar¹ and Anne Jaya Ramya¹

DOI : http://dx.doi.org/10.13005/ojc/290355

A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of cinitapride and pantoprazole simultaneously in combined dosage form. The two components cinitapride and pantoprazole were well resolved on an isocratic C18 column, utilizing a mobile phase composition of acetonitrile: phosphate buffer (50:50, v/v, pH 6.8) at a flow rate of 1.0 mL/min with UV detection at 281 nm. The retention time of cinitapride and pantoprazole were 4.5min and 5.4min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines.  Linearity for cinitapride and pantoprazole were found in the range of 1.5-10.5μg/ml and 20-140μg/ml, respectively. The percentage recoveries for cinitapride and pantoprazole ranged from 97.9-103.44 % and 98.9-103.1%, respectively. The proposed method could be used for routine analysis of cinitapride and pantoprazole in their combined dosage forms.

Cinitapride;Pantoprazole;RP-HPLC

[ View HTML Full Text]