Abstract

A. Vijayalakshmi and J. Thamarai Selvi

A biosensor based on Calcium lactate enzyme was developed for the determination of calcium ions on the activity of the enzyme. The biosensor had a linear relation to calcium concentration 1M to 1x10-4M and used without an internal solution to give a near–Nernstian responses in with detection limits of the order of 10-4M. The stable potentiometric signals are obtained within a short time period of 1 minute. The effect of pH has been studied to found a better response range. The potentiometric selectivity coefficient values were evaluated. The sensors have also been used for the determination of concentration of Ca2+ in milk.

Calcium ion selective electrode; Glutaraldehyde (GA); Potentiometer; Cross linking; Immobilization

[ View HTML Full Text]