Abstract

Swati D. Bhende^1*, Murali Balaram Varanasi², Konde Abbulu³, M. Divya Swetha¹, V. Shravanthi¹, J. Karuna Kumari¹ and  T. Shayamala¹

A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Sitagliptin phosphate and Metformin hydrochloride in marketed tablet formulations is developed. The determination was carried out on an XTerraC8 (4.6 × 100 mm, 3 µm) column using a mobile phase of pH-9 phosphate buffer solution: acetonitrile: methanol in the ratio of 35:45:20. The flow rate was 0.6 ml/min. The analyte was monitored using UV detector at 260 nm. The retention time for Sitagliptin phosphate was 3.056 mins and for Metformin hydrochloride 2.420 mins. Linearity of Sitagliptin and Metformin were found in the range of 50 ppm to 150 ppm. Percentage recoveries were obtained in the range of for Sitagliptin 99.5% and for Metformin 100.4%. The proposed method is precise, accurate, selective, robust, reproducible and rapid for the simultaneous estimation of Sitagliptin phosphate and Metformin hydrochloride in tablet dosage forms.

Sitagliptin phosphate; Metformin HCl; RP-HPLC; UV

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