Abstract

Mohammad Bagher Pashazanousi^1*, Hossein Ali Mashayekhi² and Mohammad Rezaee³

Dispersive liquid-liquid microextraction (DLLME) coupled with high-performance liquid chromatography (HPLC) – UV detection was applied for the extraction and determination of abamectin (avermectin (B1b) and avermectin (B1a)) in fruit juice samples. An appropriate mixture of ethanol (as the disperser solvent) and carbon tetrachloride (as the extraction solvent) was injected rapidly into a sample containing abamectin. After extraction, phase separation was performed by centrifuging the mixture and the sedimented phase was analyzed by HPLC-UV. Some effective parameters on the extraction, such as types and volumes of extractant and disperser solvents and salt effect were investigated and optimized. Under the optimum conditions, (extractant solvent: carbon tetrachloride, 30.0 µL; disperser solvent: ethanol, 1.0 mL and without salt addition), the calibration graphs were linear in the range of 2.5 - 500 µg L-1 and 1.0 – 500 µg L-1 with the detection limits of 0.8 µg L-1 and 0.3 µg L-1 for B1b and B1a in fruit juice samples, respectively. The relative standard deviation (R.S.D., n = 5) for the extraction and determination of 50.0 µg L-1 of B1b and B1a in the fruit juice samples were 9.2 and 7.8%, respectively. The results show that DLLME is a very simple, rapid, sensitive and efficient analytical method for the determination of abamectin in fruit juice samples and satisfactory results were obtained.

Dispersive liquid-liquid microextraction; Abamectin; High-performance liquid chromatography; Fruit juice samples

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