Abstract

Vinay Pandit*, Yadav Vivek Ramshankar and Sarasija Suresh

A rapid, sensitive, and reproducible microtiter plate assay for the determination of Metformin in plasma and water was developed. Plasma or water samples (100 μl aliquots) were prepared by the addition of acetonitrile (1 ml) and then briefly vortex-mixed and centrifuged at 12000 rpm for 20 minutes. Supernatant was taken in a fresh eppendorf tubes and to this 1 ml of dichloromethane was added. Tubes were vortexed and centrifuged at 12000 rpm for 20 minutes. Supernatant was collected and an aliquot 50 μl of each sample was transferred to a 384-well microtiter plate and read spectrophotometrically at 246 nm. Calibration curves were linear over the concentration range of 0.5–4 mg/ml or 0.025–0.4 mg/ml for plasma and water, respectively; correlation coefficients (r2) were >0.9969. The intra and inter-day coefficients of variation in plasma, water were <15%. This method is well suited for the rapid analysis of large s of samples and is currently being used for in vitro investigations of metformin.

Metformin; Microtiter plate assay; Plasma

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