Abstract

K. C. Srinivasa Murthy

A sensitive, reproducible and accurate spectrophotometric method has been developed for determining amitriptyline hydrochloride (ATH) from its pharmaceutical formulation based on solvent extraction into chloroform of the product formed with 1, 2-naphthoquinon-4-sulphonate. The reaction and extraction was complete and quantitative at pH 1.3. The Beer’s law of yellow colored species is obeyed in the range of concentration of 2 – 18 µg ml×^-1 at the maximum absorption of 450 nm. The molar absorptivity and Sandell’s sensitivity values were found to be of 3.2382 x 10³l mol^-1cm-× and 0.9615 µg cm^-2 respectively. The results compare favourably with those of official methods. The method is successfully employed for the determination of ATH in pharmaceutical preparations.

Amitriptyline hydrochloride; spectrophotometry; 1; 2-naphthoquinon-4-sulphonate

[ View HTML Full Text]