Abstract

Mamta Shrivastava, Mukul Sharma and Afraim Oty

A spectrophotometric method has been developed for the determination of penicillamine, using Ru (III) as complexing reagent .The complex formed gives a maximum absorbance at 545 nm resulting in an orange coloured stable complex formed within 20 minutes with molar absorption coefficient 2.1× 10⁴ L mol^-1 cm^-1. The complex obeys Beer‘s law in the range of 0.099 ppm to 1.399 ppm. Stoichiometry of the complex is confirmed by Mole ratio method as well as Job‘s method of continuous variance and M: L (metal: ligand) ratio was found to be 1:2.In addition, on the basis of Elemental analysis, FTIR, H¹ NMR ESR, RAMAN spectral data a plausible structure of mononuclear Ru (III) complex of penicillamine {Ru PAM₂ (H₂O} has been reported. Thus confirming the bonding of Ru (III) ion with sulphydryl and amino group of penicillamine.The formed complex was further screened for the antibacterial activity against several bacteria and the results are compared with the activity of penicillamine.

Penicillamine; Spectrophotometric; Antibacterial and ruthenium (III) chloride

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