Abstract

Zuhrina Masyithah

DOI : http://dx.doi.org/10.13005/ojc/320310

Response Surface Methodology (RSM) is successfully used to obtain the optimum conversion of oleic acid on the synthesis of fatty amide surfactants from oleic acid and diethanolamine by immobilized lipase. The central composite design is adopted to determine the optimum level of three study variables, i.e. enzyme amount, substrate molar ratio, and temperature. The model resulted in this study is suitable to represent the interaction among three variables and also their interaction to the conversion of oleic acid. The influence of substrate molar ratio is more significant among others. The prediction of the statistical model shows that the maximum conversion of oleic acid would be 78.01% at the optimal condition of 59% (wt/wt OA) enzyme amount, 1/1 to 3/1 molar ratio of diethanolamine to oleic acid and 6065^oC temperature. The coefficient of determination (R² = 0.9897) shows a high correlation between predicted and experimental values.

Surfactants; amidification; fatty amide; immobile lipase; Response Surface Methodology

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