Abstract

Sayanna Kondamadgu and Venkateshwarlu Gandu*

DOI : http://dx.doi.org/10.13005/ojc/320179

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Dobutamine hydrochloride, Lomefloxacin hydrochloride, Sildenafil citrate, Ramipril, Telmisartan based on their reactivity towards N- bromosuccinamide (NBS). The method of each drug depends upon oxidation of drugs by NBS (Excess) and estimating the amount of un-reacted NBS by Methyl Orange dye at λmax 508nm. The calibration curves obeyed Beer’s law over the concentration range of 5-38 µg mL^-1 (DOB), 5-50 µg mL^-1 (LOM), 4-28 µg mL^-1 (SIL), 2-14 µg mL^-1 (RAM) & 6-42 µg mL^-1 (TEL). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of ICH.

methyl orange; NBS; quantification; spectrophotometry; validation

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