Abstract

Raveendra Babu¹, A. Lakshmana Rao^2* and J. Venkateswara Rao³

DOI : http://dx.doi.org/10.13005/ojc/320178

A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Paracetamol and Cefixime in rabbit plasma using Cefaclor  as internal standard. Evaluation of the content of drugs were done by employing a  mixture of Phosphate buffer (p^H 6.4) and Acetonitrile (80:20, v/v) as the mobile phase and  measure the absorbance at 245nm for Paracetamol and Cefixime. Retention time was  established to be 3.618min for Cefaclor, 4.608min for Paracetamol and 5.914min for Cefixime. The results shown that the analytical technique furnished here establishes  acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.

Paracetamol; Cefixime, Bioanalytical; Simultaneous; Development

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