Abstract

Mamta Sharma¹ and Minakshi Sharma^*

DOI : http://dx.doi.org/10.13005/ojc/320158

Galctose oxidase nanoaggregates have been prepared by chemical desolvation method involving the crosslinkng agent glutaraldehyde. These enzyme nanoagregates have been characterized by transmission electron microscopy(TEM), UV visible spectroscopy, Fourier transform infrared spectroscopy (FTIR). TEM  reveals the globular spherical  nanostructured form upto the range of 20nm. UV visible spectroscopy of galactose oxidase nanoaggregates shows maximum absorption peak at 237nm. FTIR spectra obtained a C-N bending at 1634cm^-1 due to glutaraldehyde crosslinking. NP aggregates were more stable, active and had a higher shelf life than that of free enzyme and  reused many times.

Nanoaggregates; Spectroscopy; structure; Galactose oxidase; microscopy

[ View HTML Full Text]