Abstract

Li Wenbin and Li Zengbo^*

DOI : http://dx.doi.org/10.13005/ojc/320112

The present study has been aimed to assess biological stability of the antagonism actinomycete SC-04 fermentation fluid isolated from the Qinghai-Tibet Plateau soil of China. Aspects on antimicrobial effect to pathogenic fungi and polarity of antimicrobial substance have also been characterized. The results showed that the fermentation fluid of antagonism actinomycete SC-04 was stable under room temperature less than 90 d, it had a remarkable thermal stability under 70°C less than 30 min or under 100°C less than 10 min, and also had a pH stability among 4.0 and 12.0. Moreover, it was UV stable if irradiated less than 30 min. In addition, this fermentation broth was soluble in water and also in solvent, and could be extracted using ethyl acetate. Furthermore, the extract of SC-04 fermentation fluid by ethyl acetate had relative widely antimicrobial spectrum to the most pathogenic fungi, especially for Alternaria solani and Phytophthora capsici. Thus, the SC-04 fermentation fluid could be used for the production of antibiotics and develope for biological pesticide.

Actinomycete SC-04; Fermentation; Antimicrobial activity

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