Abstract

Alireza Jafari¹ , Masood Ghane² *, Mehrdad Sarabi³ and Farhod Siyavoshifar⁴

With the emergence of microbial organisms resistant to multiple antibiotics, especially, in the many hospitals, kindergartens and public place; in the present time, many researchers are attempting to develop new. The aim of this research is to comparsion antibacterial activity of mono-metallic with composite nanocrystales, against Escherichia coli(PTCC 1533), Salmonella galinarum (PTCC 1510), Staphylococcus aureus (PTCC 1113), Pseudomonas aeroginosa (PTCC 1310), and Bacillus subtilis (PTCC 1023), with and without sonication, for the first time.Mono-metallic with composite nanocrystales, are synthesized via wet method and ensure with oxalate decomposition in high temperature (5000c). FT-IR, XRD, and SEM were used for determination of spectroscopic, structural and morphology of samples, respectively. Also, the nanoparticles were digested and analyzed by ICP-AES for determining the presence of residual chemical element in the nanoparticles, after sonication. Bacterial sensitivity to nanocrystales, with and without sonication, were commonly tested using by disc diffusion test and agar dilution test, also with determination of minimum inhibitory concentration(MIC), and minimum bactericidal concentration(MBC).The particles size were less of 30 nm, approximately. The diameter inhibitory zone showed that all of the strains, have got more Sensitivity against ZnO. Antibacterial activity of CuO nano-crystales were weaker than ZnO and ZnO/CuO. Furthermore, we reported that the least value of MIC, against all strains, dependent to the ZnO nanoparticles, wheras the great value of MIC dependent to the CuO nanoparticles. Also, the great value of bactericidal effects displayed to ZnO nanocomposites.This study shows us that synthesise of mono-metallic and composite nanocrystales, with oxalate decomposition method is simple and so useful. Despite the fact, we confirmed that utilized of the ultrasonic vibration were futile, entirely. It is worthy noting that, the ZnO mono-metallic nanocrystales, had got very effective against all of the strains wheras, the CuO/ZnO nanocrystales had not great antimicrobial agent against all of the strains and combination of zinc oxide and copper oxide nanocrystales, gave decline their bactericidal effect.

Synthesis; Antibacterial Properties; CuO/ZnO Nanocrystales

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