A Convenient Method for the Estimation of Cyanide in Water and Biological Fluids

P. K. Gutch, Sri Prakash, S.R.S. Sachan, R.C. Malhotra² and Shashi Prabha²

¹Department of Chemistry, Defence Research and Development Establishment, Gwalior, India. ²School of Studies in Chemistry, Jiwaji University, Gwalior 474 002, India.

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ABSTRACT:

A new sensitive spectrophotometry method for the quantitative estimation of cyanide in water based on reaction of copper sulphate and o-toluidlne with cyanideion to form o-toluidine blue is reported. This method has been successfully extended to estimate plasma cyanide levels in rats in vitro and in vivo after cyanide administration. A linear plot is obtained at 624 nm in the concentration range 6-50 ng ml-1.

KEYWORDS:

spectrophotometry method; cyanide; copper sulphate; o-toluidlne

Article Publishing History

Received on: April 22, 1996