Purification and Identification of 35kda Heat Stable Protein

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A heat stable protein (hsp) has been purified to homogeneity from sheep liver extract by heating to 95c followed by anion-exchange chromatography on DEAE-cellulose (DE-52). The purified protein showed a single band when examined by gel electrophoresis. The molecular weight determined by SDSpage was found to be approximately 35KDa. The presence of this protein was also demonstrated in whole cell homogenate, heat stable and nuclear fraction.

Hsp; DEAE; SDSpage