Purification of Hsc70 from Liver Tissue

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A 70KDa protein has been purified by anion exchange chromatography on (DE-52).Molecular weight of protein was also determined by employing 10% SDS-page with phosphorylase b(Mr 92,500) BSA (Mr 68000) ovalbumin(Mr 43,000) chymotrysinogen(Mr 27,000) and Myoglobin(Mr17200) as the marker proteins.After electrophoresis gel was stained with 0.25% CBB for 4 hour and were then destained.

SDS DEAE; CBB