Application of Ninhydrin and Ascorbic Acid for The Determination of Cefpodoxime Proxetil in Pharmaceutical Formulations
1Department of Chemistry, St. Theresa’s College for Women, Eluru India.
2Department of Chemistry, Acharya Nagarjuna University P.G. Center, Nuzvid, Krishna India.
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A simple and sensitive spectrophotometric method by exploiting the importance of the condensation reaction of Ninhydrin1 indane-1,2,3- trione hydrate and Ascorbic acid2-4 for the assay of Cefpodoxime proxetil has been described (CEFP), in bulk form and dosage forms. The ninhydrin reaction is one of the most useful reactions of a-amino acids. Ninhydrin along with a reducing agent has been used as a chromogenic reagent. In the present method, the drug CEFP which possesses -NH2, when heated with ninhydrin in the presence of ascorbic acid afforded a blue violet color product. The absorption maximum was exhibited at 590nm. Regression analysis of Beer’s law plots showed good correlation in the concentration range 10-60 μg/ml.
KEYWORDS:Cefpodoxime proxetil; Ninhydrin; Ascorbic acid; Spectrophotometry
