Spectrophotometric Determination of Certain B-Lactam and Aminoglycoside Antibiotic Drugs Using 2,3-Dichloro-5,6-Dicyano-R-Benzoquinone

Subbiah Thangadurai¹*, Sudhir Kumar Shukla²**, Yerramilli Anjaneyulu³#

¹Department of Geology and Mining, Guindy, Chennai- 600 032 (India) ²Central Forensic Science Laboratory, BPR and D, Hyderabad- 500 028 (India) ³Centre for Environment, IPGS and R, JNT University, Hyderabad- 500 028 (India)

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ABSTRACT:

The aim of this study was to develop a spectrophotometric method for the determination of certain b-lactam and aminoglycoside antibiotic drugs in pure and forensic samples. The method is based on the certain of ampicillin sodium, amoxycillin sodium, amikacin sulphate, benzylipenicillin, benzathine penicillin, ceftriaxone sodium, gentamicin sulphate, streptomycin sulphate and neomycin sulphate as n-electron donor with 2,3-dichloro-5, 6-dicyano-p-benzoquinone (DDQ) as p-acceptor to yeild highly coloured radical anion. The coloured products are quantified spectrophotometrically at 326nm, 380nm, 480nm. The optimum reaction conditions and other analytical parameters have been evaluated for accuracy and precision.

KEYWORDS:

Spectrophotometry; antibiotic drugs