Hplc Analysis and Changes in Carbohydrate Content in Potato Tubers Stored at Ambient Temperature

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A sensitive, rapid and reliable method has been developed for the quantification of fructose, glucose and sucrose content of potato tubers, during storage for a period of twelve weeks. The separation was achieved on a μ-Bondapak-NH2 column using RCN: H2O (80:20 v/v) mobile phase at a flow rate of 2.5 ml/min. and refractive index detector. Potato tubers accumulated considerable amount of total sugars, when stored under ambient conditions from March to June, 1997 (monthly average temperature between 24 to 36°C). Sucrose was the main component of reducing sugar accumulated during the storage.

Carbohydrate content; HPLC analysis; ambient temperature