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A New Kaempferol Glycoside with Antioxidant Activity from Chenopodium ambrosioides Growing in Egypt

Mosad Ahmed Ghareeb1*, Amal Mohamed Saad1, Allia Mahmoud Abdou1, Laila Abdel-Ghany Refahy1 and Wafaa Sabry Ahmed1,2

1Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar 12411, Imbaba (P.O. Box 30), Giza, Egypt.

2Department of Chemistry, College of Science and Arts, Sajir, Shaqra University, Kingdom of Saudi Arabia.

Corresponding Author E-mail: m.ghareeb@tbri.gov.eg

DOI : http://dx.doi.org/10.13005/ojc/320626

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ABSTRACT:

The current study aimed to identify the chemical constituents of Chenopodium ambrosioides (Linn.), and the assessment of the in vitro antioxidant activity of the different extracts and pure isolates. Methods: The antioxidant activity was estimated via free radical scavenging and Phosphomolybdenum assays. Structure elucidation of pure compounds was achieved via UV, IR, 1H & 13C-NMR, 1H-1H COSY, HMQC, and HMBC, spectroscopy. Bioassay-guided fractionation and isolation of the n-butanol fraction led to the isolation of a new kaempferol glycoside namely; kaempferol 3-O-α-L-1C4-rhamnosyl-(1'''→2'')-β-D-4C1-xylopyranoside (1), together with five known compounds identified as; kaempferol 3-O-α-L-1C4–rhamnopyranoside (afzelin) (2), kaempferol 7-O-α-L-1C4–rhamnopyranoside (3), caffeic acid (4), 1,2-benzopyrone (coumarin) (5), and kaempferol (6). Compound (1) showed in vitro antioxidant activity of SC50 12.45 μg/ml, compared to ascorbic acid (AA) with SC50 of 7.50 μg/ml. It can conclude that the leaves of C. ambrosioides can be used as promising natural antioxidants agents.

KEYWORDS:

Chenopodiaceae; Chenopodium ambrosioides; kaempferol 3-O-α-L-1C4-rhamnosyl-(1'''→2'')-β-D-4C1-xylopyranoside; Antioxidant; DPPH; 2D-NMR

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Ghareeb M. A, Saad A. M, Abdou A. M, Refahy L. A. G, Ahmed W. S. A New Kaempferol Glycoside with Antioxidant Activity from Chenopodium Ambrosioides Growing in Egypt. Orient J Chem 2016;32(6).


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Ghareeb M. A, Saad A. M, Abdou A. M, Refahy L. A. G, Ahmed W. S. A New Kaempferol Glycoside with Antioxidant Activity from Chenopodium Ambrosioides Growing in Egypt. Orient J Chem 2016;32(6). Available from: http://www.orientjchem.org/?p=26315


Introduction 

The genus Chenopodium constituted of approximately 120 species, 45 of which are known to be distributed all over the world, and nine of which are distributed in Egypt1,2. Different species belonging to this genus showed numerous pharmacological properties3,4. Also, the previous chemical investigations carried out on this genus revealed the presence of flavonoids5-7, terpenes8, sesquiterpene pygmol9, xyloside10, coumarins11, and essential oils12. Chenopodium ambrosioides Linn (Family Chenopodiaceae) is native to temperate America13. For a long time C. ambrosioides was used traditionally for solving many health problems i.e., inflammation, diabetes, parasites, cough, typhoid, and influenza14,15. Reviewing the literature revealed that the different parts of C. ambrosioides showed numerous biological activities i.e., antimicrobial16,17, cytotoxicity18, antioxidant19, larvicidal20, antidiabetic4, antiparasitic21, antiviral22, and molluscidal23. Moreover, from the phytochemistry point of view C. ambrosioides is known to contain secondary metabolites i.e., saponins24, terpenoids2,25,26, flavonol glycosides27,28, flavone glycosides29, and chenopodiumamines & chenopodiumoside30. The accumulation of the reactive free radicals in our bodies led to phenomena known as oxidative stress which represent the imbalance between the endogenous antioxidant molecules in our bodies and the extent of such species. Consequently, any excess amounts of such species led to harmful effects, and in such case should be fixed via supplying the human body by external antioxidants agents in the form of nutrients or food additives19. Therefore, the aims of our current research work were to evaluate the in vitro antioxidant activity of C. ambrosioides leaves growing in Egypt, accompanied by the chromatographic separation and identification of its bioactive pure isolates.

Materials and Methods

Plant materials 

Leaves of Chenopodium ambrosioides Linn (Family Chenopodiaceae) were collected from Giza Governorate, Egypt in February 2015. The plant was identified by Dr. Threase Labib, Consultant of Taxonomy at the Ministry of Agriculture. A voucher specimen (Ca-2015) was kept at the Herbarium of our department.

Chemicals and apparatus

Melting point (uncorrected) was recorded using SMP3 Stuart Scientific device (UK). Spectral analyses were measured via Varian Mecauy 300 MHz spectrometer (1H, 300 & 13C, 75 MHz, in DMSO-d6). Chemical shifts (δ) were expressed in ppm and coupling constant (J) in Hertz. The absorbance measurements were recorded using Camspec (Model M550) double beam scanning UV/Vis spectrophotometer. Infrared spectra were determined in Fourier Transform Infrared Spectrometer (FT/IR)-6100 JASCO. Sephadex LH-20 (Uppsala, Sweden), paper chromatography (PC) was carried out on Whatman No. 1 and No. 3 paper sheets (57 x 46 cm) (S1, n-butanol: acetic acid: H2O; 4:1:5 upper layer & S2, water: acetic acid; 85:15) (Maidstone, England), while thin layer chromatography (TLC) was performed over pre-coated silica plates (GF254, Merck) (S3, CHCl3: MeOH: H2O; 7:3:0.5).

Extraction and fractionation

The dried leaves (2.5 Kg) of C. ambrosioides were extracted via percolation in 70% methanol (5 L) at room temperature 25±2°C, and then repeated till complete extraction process. The combined extracts were concentrated via Rotatory evaporator at 45°C, to afford a dark brown residue (230 g) from 70% methanol extract, then was dissolved in petroleum ether (60-80°C) (2 L) to remove the lipoidal materials i.e., fats and sterols, to afford a semi oily residue (6.06 g). The defatted 70% methanol (210 g) was suspended in distilled water and then successively fractionated with methylene chloride (2 L), ethyl acetate (2.5 L), n-butanol (3 L), to afford methylene chloride (31.89 g), ethyl acetate (3 g), n-butanol (27 g), and water (126 g) dry fractions. The resulting fractions were chromatographically compared via PC using solvent systems (S1& S2), which guide us to select the n-BuOH fraction for further chromatographic separation.

Antioxidant assays

Free radical scavenging antioxidant activity assay 

The free radical scavenging antioxidant activity of different fractions and pure compounds was evaluated according to the reported method31.

Phosphomolybdenum assay

The total antioxidant capacity was evaluated by the reported method with some minor modifications32

Statistical analysis

The antioxidant data were presented as mean±standard deviation (S.D.) of triplicates (n=3) using SPSS 13.0 program (SPSS Inc. USA).

Chromatographic isolation of n-BuOH fraction

Twenty five grams of the n-butanol fraction were subjected to chromatographic isolation using polyamide column chromatography (CC.) (100 X 6 cm, 300 gm). A gradient elution was started with 5% MeOH and the polarity was gradually increased by methanol to pure MeOH at the end. Fractions (250 ml each) were collected, concentrated and examined (PC, S1&S2, 5% AlCl3 and 1% FeCl3, UV light for detection). Similar fractions were pooled according to their pattern upon paper chromatography, to afford five main fractions (1-5) matching to the flavonoids & phenolic category. Fraction 1 (3 g) was eluted with 10% MeOH, and purified over Sephadex LH-20 (CC.) to afford a yellow needles of compound 1 (40 mg) eluted via H2O: MeOH (40:60). Fraction 2 (2 g) was eluted with 20% MeOH, and purified over Sephadex LH-20 to afford compound 2 (20 mg) eluted via H2O: MeOH (30:70). Fraction 3 (3 g) was eluted with 30% MeOH, and purified over Sephadex LH-20 to afford compound 3 (25 mg) was eluted via H2O: MeOH (25:75). Fraction 4 (4 g) was eluted with 40% MeOH, was purified over Sephadex LH-20 to afford compound 4 (10 mg) and compound 5 (20 mg) were eluted via H2O: MeOH (20:80) and H2O: MeOH (15:85) , respectively. Finally Fraction 5 (2 g) was eluted with 70% MeOH, and purified over Sephadex LH-20 to afford compound 6 (15 mg) eluted via H2O: MeOH (10:90).

Complete acid hydrolysis  

The compound (3-5 mg) was refluxed using dilute hydrochloric acid HCl in MeOH at 100°C for 3 hrs, then the hydrolysate was exhaustively extracted with ethyl acetate in separating funnel. Aglycones identified via Co-PC with authentic aglycone sample. The aqueous phase was neutralized with 5% sodium bicarbonate and used for investigation of the sugar moieties via Co-TLC with authentic sugar markers33.

Results and Discussion

In vitro antioxidant activities (DPPH, TAC)

In the current study, the Chenopodium ambrosioides leaves were examined for their antioxidant activities using 2,2′ diphenyl-1-picrylhydrazyl radical (DPPH·) and Phosphomolybdenum assays. The results in Table 1 revealed that η-butanol extract exhibited a potent activity with SC50 value 2.98 μg/ml, which nearly twice the value of standard ascorbic acid 7.50 μg/ml. While, the ethyl acetate extract exhibited activity with SC50 value 16.48 μg/ml, followed by a moderate activity of the 70% methanol extract with SC50 value 55.33 μg/ml, while the remaining teste extracts (petroleum ether and methylene chloride) showed very weak activity >100 μg/ml. Furthermore, in the phosphomolybdenum assay the n-butanol extract was also the most potent of TAC (554.54 ± 2.27 mg ascorbic acid equivalent/g dry extract), accompanied  by ethyl acetate and 70% MeOH of TAC (418.93 ± 2.62), and (341.66 ± 1.10) respectively, however the weak activity was recorded with the remaining fractions CH2Cl2 (159.84 ± 1.29), and pet. ether (118.93 ± 1.31) mg ascorbic acid equivalent/g dry extract) (Table 1). The results obtained can serve as a significant indicator for the antioxidant activity of the η-butanol extract which prompted us to subject it for further chromatographic isolation to identify its chemical constituents which may be responsible for such activity. Reviewing the literature revealed, several Chenopodium species were investigated for their in vitro antioxidant activities, i.e., Amri et al. (2015) reported that the antioxidant activity of the 80% methanol extract of Moroccan Chenopodium ambrosioides equal to 73.80%34. In addition, the 80% MeOH of C. ambrosioides leaves growing in Egypt showed superoxide anion scavenging activity of 55.78% and iron chelating activity 73.37%35. Moreover, Barros et al. (2013) reported that the activity of the methanolic extract of Portuguese C. ambrosioides was EC50 was equal to 0.62 mg/ml19. Furthermore, the activity of the methanolic extract of C. ambrosioides growing in Yemen was 31.9% at 100 mg/ml36.  Actually,  no reports are accessible on the total antioxidant capacity of the C. ambrosioides.

Table 1: Antioxidant activities of the different fractions of C. ambrosioides

Sample

DPPH free radical scavenging activity SC50g/ml)a

Total antioxidant capacity (mg ascorbic acid equivalent AAE /g dry extract)b

70% MeOH

55.33 ± 0.78

341.66 ± 1.10

Pet. ether

>100

118.93 ± 1.31

CH2Cl2

>100

159.84 ± 1.29

EtOAc

16.48 ± 0.16

418.93 ± 2.62

n-BuOH

2.98 ± .011

554.54 ± 2.27

Ascorbic acid

7.50 ± 0.32

 ̶

aSC50: concentration from sample required for scavenging of 50% of radical.  

bTotal antioxidant capacity (TAC) was evaluated by the phosphomolybdenum assay in mg ascorbic acid equivalent AAE /g dry extract.

Identification and characterization of the isolated metabolites

In the current study, the chromatographic isolation and purification of the n-butanol fraction of C. ambrosioides resulted in the separation of six compounds (Figures 1, 2). The new compound was identified on the basis of 1D & 2D-NMR as; kaempferol 3-O-α-L-1C4-rhamnosyl-(1”’→2”)-β-D-4C1-xylopyranoside (1), as well as five known compounds were identified as; kaempferol 3-O-α-L-1C4–rhamnopyranoside (afzelin) (2), kaempferol 7-O-α-L-1C4–rhamnopyranoside (3), caffeic acid (4), 1,2-benzopyrone (coumarin) (5), and kaempferol (6).

Structural elucidation

Compound (1)

Was obtained as yellow needles, mp 208-210oC, Rf-values 0.56 in (S1), and 0.75 in (S2). UVλmax nm: MeOH 267, 301, 356; +NaOMe 275, 336sh, 400; + AlCl3 275, 305sh, 356, 412; + AlCl3 ± HCl 278, 302sh, 349sh, 409; + NaOAc 274, 314sh, 396; + NaOAc ± H3BO3 271, 308sh, 362 nm. Acid hydrolyses of 1 gave xylose & rhamnose in the aqueous phase and kaempferol in the organic phase (Co-PC). 1HNMR of the aglycone showed two characteristic spin coupling systems, each of two doublets in the aromatic region, for 4′-hydroxy B-ring and 5, 7-dihydroxy A-ring of a kaempferol moiety. The 1st system was described as AX of two ortho doublets  at δ 7.80 and 6.90 ppm for H-2’∕6′ and H-3’∕5′, respectively, while the 2nd system was of two meta doublets at 6.78 and 6.45 ppm of H-8 and H-6, respectively.  1HNMR also showed a β-anomeric proton signal of inner xyloside moiety at δ 5.38 ppm and CH3-6”’ as doublet at 1.11 ppm of terminal rhamnosyl moiety. Two anomeric proton signals were assigned at δ 5.38 (xyloside) and 5.15 (rhamnoside) ppm, in the 1HNMR spectrum through their direct one bond coupling in the HMQC spectrum with their own anomeric carbon signals at 101.5 and 102.0 ppm, respectively (Table 2). The 3-O-subistituted kaempferol moiety was confirmed from its characteristic 13 resonances in the 13CNMR spectrum as it was interpreted above, specially signals at 130.8 and 115.4 ppm of C-2’∕6′ and 3’∕5′ intrinsic for 1,4-disubstituted B-ring of kaempferol aglycone. Glycosidation at OH-3 was proved by the relative upfield shift of C-3 to 134.7 (Δ≈-3-4 ppm) and downfield shift of C-2 at 156.2 ppm. The connectivity of the xyloside moiety to C-3 of the aglycone was further proved by the correlation peak of H-1″ (δ 5.38) and C-3 (δ 134.7 ppm) in HMBC spectrum. Similarly the downfield shift of C-2″ (79.2 ppm) explained the attachment of the rhamnosyl moiety at C-2″ of xylose. The interglycosidic linkage was also assigned as (1′”→2”) on the basis of the three bond correlation peak between H-1′” (δ 5.15 ppm) and C-2″ (δ 79.2 ppm) in the HMBC spectrum. The sugar moieties were deduced to have β-4C1-, or α-1C4-pyranose stereo structure in the case of the xylosyl and rhamnosyl moieties, respectively, on the basis of the J-values of the anomeric protons and δ-values of their 13CNMR resonances (Table 2).  All other 1H and 13C resonances were also confirmed by the 1H-1H-COSY, HMQC and HMBC spectra and by comparison with previously reported data for structurally related compounds7. Thus, compound 1 was identified as kaempferol 3-O-α-L-1C4-rhamnosyl-(1”’→2”)-β-D-4C1-xylopyranoside (Figures 1, 2).

Compound (2)

Was obtained as pale yellow powder, mp 181-183oC. Rf-values 0.69 in (S1), and 0.58 in (S2). IR (KBr cm1): showed peaks at 3350, 1658, 1609, 1510, 1456, 1369, 1308, 1028, 823 cm−1. It showed similar chromatographic behavior, chemical and NMR spectral data to kaempferol except for the NMR spectral data assignable to the sugar moiety. Acid hydrolysis of compound 2 afforded rhamnose in the aqueous phase and kaempferol in the organic phase. The 1HNMR at δ 5.54 (assignable to α-anomeric protons as brs) and at 1.05 (for CH3-6”) were characteristic for α-L-rhamnopyranosyl moiety at OH-3. Compound 6 showed six 13C- resonances of O-rhamnopyranoside moiety among which C-4”, C-3”, C-2” and   C-5” of δ- values around 70 ppm and CH3-6” as the most upfield signal at 17.4 ppm. Glycosidation at OH-3 was proved by the relative upfield shift of C-3 to 134.9 (Δ≈-3-4 ppm) and downfield shift of C-2 at 157.7 ppm. Assignment of all other 13C resonances was proved by their comparison with the reported data in the literature37. Hence compound 6 was identified as kaempferol 3-O-α-L-1C4 –rhamnopyranoside (afzelin) (Figures 1).

Compound (3)

Was obtained as a yellow powder, mp 171-172oC. Rf-values 0.64 in (S1), and 0.55 in (S2). IR (KBr cm1): showed peaks at 3340, 1655, 1620, 1515, 1465, 1350, 1025, 830 cm−1. The aglycone moiety showed very similar chromatographic behavior, chemical and NMR spectral data to kaempferol aglycone. Acid hydrolysis of compound 3 afforded rhamnose in the aqueous phase and kaempferol in the organic phase. NMR spectral data of 3 resembled that of compound 2 (afzelin) for aglycone and sugar moiety. However in compound 3 the rhamnose moiety attached to C-7 instead of C-3 in compound 2, which confirmed from the downfield shift of both H-8 and H-6 to 6.4 and 6.10 ppm, respectively. All other NMR resonances assigned through a comparison study with the previously published data38. Therefor compound 3 was identified as kaempferol 7-O-α-L-1C4–rhamnopyranoside (Figures 1).

Compound (4)

Was obtained as a pale yellow amorphous solid, mp 225-227°C, Rf-values 0.54 in (S2), and 0.63 in (S3). It showed bluish-grey colour with FeCl3 and blue under long UV light. IR (KBr cm1): showed peaks at 3430 (Ar-ring, OH), 3235 (Ar-ring, -C-H), 2915 (aliphatic –C-H), 1650 (-C=O), 1625 (-C=C), 1619, 1520 and 1449 (Ar-ring, -C=C), 1250 and 1296 (ether bond of carboxylic group), and 1193 cm-1 (hydroxy bond of carboxylic group). 1HNMR spectra (300 MHz, DMSO-d6) showed the existence of three characteristic signals of aromatic moiety (ABX system) at δ 7.01 (1H, d, J= 2.1 Hz, H-2), 6.96 (1H, dd, J= 8.1, 2.1 Hz, H-6), 6.76 (1H, d, J= 8.1 Hz, H-5), & two signals for cinnamoeyl moiety 7.42 (1H, d, J= 15.9 Hz, H-7), and 6.18 (1H, d, J= 15.9 Hz, H-8) ppm. The mentioned data of compound 4 were matched with the previous reported data of 3-(3,4-Dihydroxyhenyl)-2-propenoic acid (caffeic acid)39 (Figures 1).

Compound (5)

Was obtained as a white fine crystal, m.p. 70°C. 1HNMR (300 MHz, DMSO-d6) revealed the presence of a characteristic signals at δ 6.47 (1H, d, J= 9.6 Hz, H-3), 8.03 (1H, d, J= 9.9 Hz, H-4), 7.29 (2H, m, H-5, 7), 7.58 (2H, m, H-6, 8) ppm40. 13CNMR (75 MHz, DMSO-d6) revealed the existence of nine signals of        9 carbon atoms assigned to; C-2 (159.84), C-3 (116.18), C-4 (144.10), C-5 (128.35), C-6 (124.38), C-7 (131.84), C-8 (116.11), C-9 (153.43), and C-10 (118.6) ppm. These spectral data were found to be in full agreement with the previous reports, therefore the compound 5 can be characterized as 1,2-benzopyrone (coumarin)41 (Figures 1).

Compound (6)

Was obtained as yellow fine crystals, mp 280-282oC, Rf-values 0.84 in (S1), and 0.38 in (S1). UV λmax nm: MeOH 264, 372; +NaOMe 263, 284, 358sh, 452; + AlCl3 262, 302sh, 363, 425; + AlCl3 ± HCl 262, 301sh, 345sh, 426; + NaOAc 263, 323sh, 385; + NaOAc ± H3BO3 258, 315sh, 368. IR spectrum (KBr cm1): showed absorption peaks at 3425 (hydroxyl), 1695 (4-oxo carbonyl), and 1650 (aromatic double bond) cm-1. Based on its chromatographic behavior and UV data compound 6 was likely to be kaempferol like skeleton. In the aromatic region there are two spin coupling systems, each of two doublets. The first one consists of two ortho doublets at 8.02 and 6.92 ppm allocated to H-2’∕6′ and H-3’∕5′ both of J=7.5 Hz, respectively of 4′-hydroxy B-ring. Whereas the second system appeared as two meta-coupled protons, which were characterized to H-8 and H-6 of 5,7-dihydroxy A-ring at δ 6.42 and 6.17 ppm both of J=1.5 Hz, respectively. 13CNMR spectrum exhibited typical 13 13C-resonances for kaempferol42. Based on these findings compound 6 was identified as 3,5,7,4′-tetra hydroxyl flavone (kaempferol) (Figures 1).

Table 2: 1H & 13C NMR spectral data (300/75 MHz-DMSO-d6), 1H-1H COSY and HMBC assignments of compound 1

Position

δH ppm1

J (Hz)2

1H-1H COSY

correlations

δC ppm

HMBC (H-C) correlations

 

 

 

 

 

 

2

157.8 (q)

3

134.7 (q)

4

178.0 (q)

5

158.0 (q)

6

6.45

1H, d, 1.8

98.6 (t)

7

161.9 (q)

8

6.78

1H, d, 1.8

94.7 (t)

9

156.2 (q)

10

105.9 (q)

1′

120.4 (q)

2′

7.80

dd, 7.2, 1.8

130.8 (t)

3′

6.90

1H, d, 8.7

115.4 (t)

4′

160.6 (q)

5′

6.90

1H, d, 8.7

115.4 (t)

6′

7.80

dd, 7.2, 1.8

130.8 (t)

1”

5.38

2”

101.5 (t)

3”, 3

2”

3”, 2”

79.2 (t)

4”

3”

4”, 2”

76.4 (t)

1”, 5”

4”

5”, 3”

70.0 (t)

6”, 2”

5”

6”, 4”

65.9 (s)

3”

6”

5”

4”

1”’

5.15

2”’

102.0 (t)

2”,3”’

2”’

3”’, 1”’

70.8 (t)

4”’

3”’

4”’, 2”’

70.5 (t)

1”’, 5”’

4”’

5”’, 3”’

73.9 (t)

2”’, 6”’

5”’

6”’, 4”’

69.5 (t)

3”’

6”’

1.11

3H, d, 6.3

5”’

18.0 (p)

4”’

1H: Chemical shifts δ in (ppm).

2Coupling constants J in (Hz).

Figure 1: Chemical structures of phenolic compounds isolated from C. ambrosioides.

Figure 1: Chemical structures of phenolic compounds isolated from C. ambrosioides 



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Figure 2: Selected HMBC correlations of compound 1

Figure 2: Selected HMBC correlations of compound 



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In vitro antioxidant activity of the isolated compounds (1-6)

The antioxidant activities of the compounds (1-6) were evaluated using free radical scavenging assay. Compound 4 exhibited potent antioxidant activity of SC50 4.20 μg/ml, followed by compound 6 of SC50 6.35 μg/ml, while the remaining compounds 1, 2, 3, and 5 showed moderate antioxidant activities of SC50 9.41, 12.45, 9.20, and 10.84μg/ml separately, with respect to ascorbic acid of SC50 7.50 μg/ml. The high antioxidant activity of 4 and 6 may be return to the presence of the characteristic structural criteria for effective free radical scavenging activity including; the presence of cinnamoeyl moiety and ortho di-hydroxy groups in case of compound 4, and also the presence of an 2, 3 unsaturated double bond, 4-oxo group (ring-C), and 5-OH (A-ring), in case of compound 643. Moreover, mono and di kaempferol glycosides 1 and 2 were less active than their aglycone 6, which may be due to steric hindrance offered by a bulky glycosidic moiety at position 3 (ring-C). On the other hand the slightly high activity of 3 than 1 and 2 due to the location of the glycosidic moiety in position 7 at ring-A which not affected on the conjugation process between ring-B and 2,3 D.B. & 4-carbonyl group of the aromatic C-ring44.

Conclusion

The current study demonstrated that n-butanol extract had the highest in vitro antioxidant activity among all tested extracts from methanolic extract C. ambrosioides. Moreover, the chromatographic isolation and purification of the most promising n-butanol extract resulted in the separation of one new compound identified as kaempferol 3-O-α-L-1C4-rhamnosyl-(1”’→2”)-β-D-4C1-xylopyranoside (1), along with five known compounds viz., kaempferol 3-O-α-L-1C4–rhamnopyranoside (afzelin) (2), kaempferol 7-O-α-L-1C4–rhamnopyranoside (3), caffeic acid (4), 1,2-benzopyrone (coumarin) (5), and kaempferol (6). It can conclude that the leaves of the Egyptian C. ambrosioides can be used as promising natural antioxidants agents. 

References 

  1. Takholm, V. In Students Flora of Egypt. Cairo University, Anglo-Egyptian Book Shop: Cairo, 1977; 107.
  2. Ahmed, A.A. J. Nat. Prod. 2000, 63, 989-991.
    CrossRef
  3. Koba, K.; Catherine, G.; Raynaud, C.; Chaumont, J.; Sanda, K.; Laurence, N. Bangladesh J. Sci. Ind. Res. 2009, 44, 435-440.
  4. Song, M.; Lee, S.; Kim, D. Phytopharmacology 2011, 1, 12-15.
  5. Pascual, J.; Gonzalez, M.S.; Vicente, S.; Bellido, I.S. Planta Med. 1981, 41, 389-391.
    CrossRef
  6. Simone, F.D.; Dini, A.; Pizza, C.; Saturnino, P.; Schettino, O. Phytochemistry 1990, 29, 369-3692.
    CrossRef
  7. Gohar, A.A.; Maatooq, G.T.; Niwa, M. Phytochemistry 2000, 53, 299-303.
    CrossRef
  8. Mata, R.; Navarrete, A.; Alvarez, L.; Pereda-Miranda, R.; Delgado, G.; Vivar, A.R.D. Phytochemistry 1987, 26, 191-193.
    CrossRef
  9. Bergeron, C., Marston, A.; Hakizamungu, E.; Hostettmann, K. Int. J. Pharm. 1995, 33, 115-1 19.
    CrossRef
  10. Dellagreca, M.; Previtera, L.; Zarrelli, A. Nat. Prod. Res. 2005, 19, 87-90.
    CrossRef
  11. El-Sayed, N.H.; Awaad, A.S.; Hifnawy, M.S.; Mabry, T.J. Phytochemistry 1999, 51, 591-593.
    CrossRef
  12. Chekem, M.S.G.; Lunga, P.K.; Tamokou, J.D.D.; Kuiate, J.R.; Tane, P.; Vilarem, G.; Cerny, M. Pharmaceuticals 2010, 3, 2900-2909.
    CrossRef
  13. Jia-liang, W.; Dan-wei, M.; Ya-nan, W.; Hong, Z.; Bing, H.; Qun, L.; Zhi-yan, Z.; Jing, F. Trop. J. Pharm. Res. 2013, 12, 929-933.
    CrossRef
  14. Ruffa, M.; Ferraro, G.; Wagner, M.; Calcagno, M.; Campos, R.; Cavallaro, L.;      J. Ethnopharmacol. 2002, 79, 335-339.
    CrossRef
  15. Yadav, N.; Vasudeva, N.; Singh, S.; Sharma, S. Nat. Prod. Radiance. 2007, 6, 131-134.
  16. Jardim, C.M.; Jham, G.N.; Dhingra, O.; Freire, M.M. J. Braz. Chem. Soc. 2010, 21, 1814-1818.
    CrossRef
  17. Shah, H.; Nisar, M.; Suhail, M.; Bacha, N. Afr. J. Microbiol. Res. 2014, 8, 2099-2104.
    CrossRef
  18. Sousa, Z.; de Oliveira, F.F.; da Conceição, A.O.; Silva, L.A.M.; Rossi, M.H.; Santos, J.S.; Andrioli, J.L. Ann. Clin. Microbiol. Antimicrob. 2012, 11, 1-7.
    CrossRef
  19. Barros, L.; Pereira, E.; Calhelha, R.C.; Dueñas, M.; Carvalho, A.M.; Santos-Buelga, C.; Ferreira, I.C.F.R. J. Funct. Foods. 2013, 5, 1732-1740.
    CrossRef
  20. Denloye, A.A.; Makanjuola, W.A.; Teslim, O.K.; Alafia, O.A.; Kasali, A.A.; Eshilokun, A.O. J. Plant Prot. Res. 2010, 50, 379-384.
  21. Salifou, S.; Daga, D.F.; Attindéhou, S.; Déguénon, R.; Biaou, C.F. J. Parasitol. Vector Biol. 2012, 5, 13-16.
    CrossRef
  22. Verma, H.K.; Baranwal, V.K. Natl. Acad.  Sci. 1983, 92, 461-465.
  23. Hmamouchi, M.; Lahlou, M.; Agoumi, A. Fitoterapia 2000, 71, 308-314.
    CrossRef
  24. Gupta, G.S.; Behari, M. J. Indian Chem. Soc. 1972, 49, 317-319.
  25. Pare, P.W.; Zajicek, J.; Ferracini, V.L.; Melo, I.S. ‎Biochem. Syst. Ecol. 1993, 21, 649-653.
  26. Kiuchi, F.; Itano, Y.; Uchiyama, N.; Honda,  G.; Tsubouchi, A.; Nakajima-Shimada, J.; Aoki, T. J. Nat. Prod. 2002, 65, 509-512.
    CrossRef
  27. Gohar, A.A.; Elmazar, M.M.A. Phytother. Res. 1997, 11, 564-567.
    CrossRef
  28. Jain, N.; Alam, M.S.; Kamil, M.; Ilyas, M.; Niwa, M.; Sakae, A. Phytochemistry 1990, 29, 3988-3991.
    CrossRef
  29. Hammoda, H.M.; Harraz, F.M.; El Ghazouly, M.G.; Radwan, M.M.; ElSohly, M.A.; Wanas, A.S.; Bassam, S.M. Rec. Nat. Prod. 2015, 9, 609-613.
  30. Song, K.; Zhang, J.; Zhang, P.; Wang, H.; Liu, C.; Li, B.; Kang, J.; Chen, R. J. Asian Nat. Prod. Res. 2015, 17, 1-9.
    CrossRef
  31. Shirwaikar, A.; Rajendran, K.; Punithaa, I.S. Biol. Pharm. Bull. 2006, 29, 1906-1910.
    CrossRef
  32. Prieto, P.; Pineda, M.; Aguilar, M. Anal. Biochem. 1999, 269, 337-341.
    CrossRef
  33. Ghareeb, M.A.; Shoeb, H.A.; Madkour, H.M.F.; Refahy, L.A.; Mohamed, M.A.; Saad, A.M.; Global J. Pharm. 2013, 7, 486-497, 2013.
  34. Amri, O.; Elguiche, R.; Tahrouch, S.; Zekhnini, A.; Hatimi, A. J. Chem. Pharm. Res. 2015, 7, 672-678.
  35. AbouZid, S.; Elshahaat, A.; Ali, S.; Choudhary, M.I. Drug Discov. Ther. 2008, 2, 286-288.
  36. Mothana, R.A.A.; Gruenert, R.; Bednarski, P.J.; Lindequist, U. Pharmazie 2009, 64, 260-268.
  37. Lee, S.Y.; So, Y.; Shin, M.S.; Cho, J.Y.; Lee, J. Molecules 2014, 19, 3173-3180.
    CrossRef
  38. Chen, C.; Hsieh, S.; Hsieh, M.; Hsieh, S.; Hsieh, T. Chin. Pharm. J. 2004, 56, 141-146.
  39. Jeong, C.; Jeong, H.R.; Choi, G.N.; Kim, D.; Lee, U.; Heo, H.J. Chin. Med. 2011, 6, 1-9.
    CrossRef
  40. Moreira, M.D.; Picanço, M.C.; Barbosa, L.C.A.; Guedes, R.N.C.; de Campos, M.R.; Silva, G.A.; Martins, J.C. Pesq. Agropec. Bras. Brasília. 2007, 42, 909-915.
  41. Luz, R.F.; Vieira, I.J.C.; Braz-Filho, R.; Moreira, V.F. Am. J. Analyt. Chem. 2015, 6, 851-866.
    CrossRef
  42. Wan, C.; Yu, Y.; Zhou, S.; Shuge, T.; Cao, S. Pharmacogn. Mag. 2011, 7, 10-108.
  43. Rice-Evans, C.A.; Miller, N.J.; Paganga, G. Free Radic. Biol. Sci. 1996, 20, 933-956.
    CrossRef
  44. Ghareeb, M.A.; Shoeb, H.A.; Madkour, H.M.F.; Refahy, L.A.; Mohamed, M.A.; Saad, A.M. Global J. Pharm. 2014, 8, 87-97.


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