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Synthesis and Characterization of 2, 3, 7, 8, 12, 13, 17, 18, Octabromo 5, 10, 15, 20 tetra phenyl porphyrin

Kalpana Raikwar

Department of chemistry, Govt.P.G.College,Guna(M.P.)473001.

Corresponding Author E-mail: kalpanaraikwar45@gmail.com

DOI : http://dx.doi.org/10.13005/ojc/310276

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Article Published : 02 Jun 2015
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ABSTRACT:

Chromium(III) porphyrin and its derivative have been synthesized by bromination and demetallation of copper tetra phenyl porphyrin.All the complexes were characterized on the basis of their elemental analysis, UV-vis spectra and magnetic moment properties.The magnetic moment suggested that the complex in the range 3.79 BM indicating an octahedral structure. The comparison of the UV- vis spectra of the free base show that substitution of Br atom at β-pyrrole position lead to greater shift than substitution of electronegative group in the phenyl ring.

KEYWORDS:

Characterization; magnetic moment; Dimethyl formamide; octa bromo tetraphenyl porphinato chromium

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Raikwar K. Synthesis and Characterization of 2, 3, 7, 8, 12, 13, 17, 18, Octabromo 5, 10, 15, 20 tetra phenyl porphyrin. Orient J Chem 2015;31(2).


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Raikwar K. Synthesis and Characterization of 2, 3, 7, 8, 12, 13, 17, 18, Octabromo 5, 10, 15, 20 tetra phenyl porphyrin. Available from: http://www.orientjchem.org/?p=8993


Introduction

Metalloporphyrins are important model compound for understanding the biological function of heme proteins1. Recent works have shown  that stability of porphyrin catalysts is greatly enhanced by substitution of   β-pyrrole and ortho position of aryl groups on porphyrin ring with different electronegative groups2. Some sterically hinderd porphyrin complexes have been found to be efficient catalyst towards alkene epoxidation, hydroxylation3 etc. Chloro tetrakis(2, 6 dichlorophenyl) porphinato iron(III) has been frequently employed as monoxygenase catalyst because of resistance of porphyrin to oxidative degradation4. Similarly a series of tetrakis (penta fluorophenyl) iron(III) complexes have been proved as efficient catalyst for selective air oxidation of alkanes5.

Much has been said about the chemistry of porphyrin complexes and the effect of various changes in their structural features. The catalytic activity of these complexes is a topic of continued research. Hence some Cr(III) complexes of tetra phenyl porphyrins having either the  β-pyrrole, position or orthophyenyl site or both position occupied by halogen atoms mainly chlorine and bromine were synthesized. The complexes have been characterized by UV-visible spectroscopy, magnetic properties, elemental analysis.

There have been much interest in the electronic structure of porphyrins and related compounds.This interest stems in part from their biological significance and catalytic properties6,7.The biologically important porphyrin derivative are all metal porphyrins have also received much attention in connection with their intrinsically interesting spectroscopic magnetic and electrochemical properties.The last decades have witnessed an explosion of experimental studies of metal porphyrins which have yielded very useful information about their electronic structure and optical spectra,but it has not always been possible to provide a well reasoned explanation of the result obtained8-11.

Metalloporphyrins is a natural choice for the optical detection of volatile organic compound because of their open co-ordination sites for axial ligation and their intense coloration12,13.Using metal contres that span a range of chemical “hardness” and ligand binding affinity,a wide range of volatile analytes might be differentiable. It has been found that porphyrin show significant solvato chromic effect,even with weakly interacting vapours14.

Experimental

The chemical components needed for the synthesis of porphyrin complexes were pyrrole, p-chloro-anil, acetylacetone, copper acetate pyridine, pottasium carbonate, sodium meta-bisulphite, anhy. sodiumsulphate, triethyl bromide, hydrochloric acid, perchloric acid, sulphuric acid, chromium trichloride, carbontetrachloride, chloroform,, dimethyl formamide, tetrahydrofuran etc.

Synthesis  of Cr(Iii) Porphyrins

Synthesis of 2,3,7,8,12,13,17,18 octabromo 5,10,15,20 tetra phenyl porphyrin and its Cr(III) derivatives

The Cr(III) derivatives  of octa bromotetra phenyl Porphyrin were synthesised by first synthesising the free base i.e. octabromotetra phenyl porphyrin [H2(OBTPP)]. The free base was then metallated with chromium, the overall procedure involved the following steps-

Preparation of Cu(TPP)15

Copper tetraphenyl porphyrin was synthesised by taking   tetra phenyl porphyrin [H2(TPP)]16 (500mg) in chloroform(100ml). Copper(II) acetate(200mg) in glacial acetic acid(50ml) was added to the above solution and the mixture was refluxed for 2hrs. The contents were concentrated to a volume of about 50-60ml and cooled to room temperature which resulted in crude copper-tetraphenyl porphyrin Cu(TPP) (about 450mg). The crude product was purified by column chromatography using neutral alumina and chloroform as eluent. On elution the unreacted tetraphenyl porphyrin was eluted out first, followed by pure Cu(TPP). The chloroform fraction containing Cu(TPP) was concentrated to obtain pure crystals of Cu(TPP)[2]. The formation of Cu(TPP) was monitored by UV-visible spectroscopy which give peaks-around 580, 541 and 417nm respectively confirming the formation of Cu(TPP) (yield=400 mg).

Figure 1 Figure 1 

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Bromination of Copper tetraphenyl porphyrin Cu(TPP)17

The copper tetraphenyl porphyrins  synthesised as above was converted  into its octabromo derivative by the addition of liquid bromine (1.2ml)in chloroform(50ml) to a solution of Cu(TPP)(0.50 g) in  chloroform:carbon tetrachloride (1:1 V/V) (500ml) in a conical flask. Bromine was added dropwise and slowly over a period of half hour, at room temperature. The contents were stirred for 4hours, followed by addition  of pyridine 2.4ml in 40ml mixture of CHCl3:CCl4 in 1:1 ratio. The addition took about half hour and stirring continued for 12hours. The bromination process was monitored by UV-visible spectroscopy to ensure complete bromination.

The  excess  bromine  was destroyed by  addition of sodium metasulphite (200ml 20% aq. solution) to the system. The organic layer was separated using a separating funnel and the solution was dried over anhydrous  sodium  sulphate. The  evaporation of solvent under reduced pressure resulted a green solid of copper octabromoteraphenyl porphyrin [Cu(OBTPP)]. The solid was dissolved in  minimum  amount  of chloroform and column chromatography was done. The first fraction coming out of the column was collected. The removal of solvent yielded copper octabromotetraphenyl  porphyrin(3) in purified form, yield (75%)

Demetallation of copper octabromo-porphyrin

Cu(OBTPP) synthesized  above  was demetallated to obtain a free base of octabromotetraphenyl porphyrin [H2(OBTPP)]. The demetallation was brought about by addition of perchloric acid(70%) 10ml to Cu(OBTPP) (250mg) in chloroform(150ml). The perchloric acid solution 70% was added to maintain a suitable pH for demetallation. The yellowish solution, obtained was stirred for about eight hours and the organic layer was separated and neutralised with 20% aqueous solution of sodium carbonate. The dark green coloured layer so obtained was separated and  dried over anhydrous sodium  sulphate. The solvent was removed leaving behind a dark green solid. This solid was dissolved in minimum quantity of chloroform and chromatographed on basic alumina column to obtain octabromotetraphenyl porphyrin[H2(OBTPP)](4).

Figure 2 Figure 2

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Chlorooctabromotetraphenyl porphinato chromium [Cr(OBTPP)Cl]

The procedure  used for the synthesis of Cr(OBTPP)Cl was as reported by Alder and co-werkers18. A typical synthesis is given below-

H2(OBTPP) (1.0gm) in 100ml DMF was taken in R.B.flask, and put to reflux so as to dissolve the ligand and after this CrCl3(0.2gm) was  added to the refluxing solution. After refluxing the contents for 10minutes a further addition of CrCl3(0.2gm) was again added. The refluxing was continued for another 10minutes and an aliquot was taken from the reaction mixture and visible spectrum was recorded using CHClas solvent. The spectrum indicated the presence of free base perphyrin H2(OBTPP) hence  CrCl3(0.2gm) was again added  to the solution and reflux continued for 10min. more. At this point a visible spectrum of an aliquot was taken from the reaction mixture which  revealed the absence of any remaining free-base porphyrin. The refluxing was stopped and the  reaction mixture was allowed to cool to room temperature. The cooled reaction mixture was poured into a flask-containing ice-cold water (500ml). The resulting  precipitate  was filtered and  washed  with small  amounts of  water  and dried in  oven for 1hr. at 1000C, the yield of crude [Cr(OBTPP)Cl] was 1.2gm.

Purification of Cr(OBTPP)Cl

Crude Cr(OBTPP)Cl (1.2gm)  was dissolved in CHCl3(150 ml) and  was loaded over alumina column(80-200 mesh). The column was  eluted using CHCl3 as eluent. The unreacted  H2(OBTPP)  was eluted as green-red  band with the solvent front and was followed by a slow-moving  green band. As the green band came off  the column it was monitored by visible spectroscopy. The band prior to Cr(OBTPP)Cl was eluted from the column was disearded. The volume of solvent was reduced to about 150ml  using a rotary evaportor. Aqueous HCl(5ml) was added to the chloroform  solution and stirring continued overnight. The solvent was  removed on a rotary evaporator. The solid was recrystallized from  chloroform / hexane and dried overnight in an oven. The yield of purified Cr(OBTPP)Cl after chromatography was 0.75gm.

Figure 3 Figure 3
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Results and Discussion

Elemantal Analysis

The porphyrin complexes of chromium(III) derivatives so synthesized were subjected to chemical analysis. The results fall within the  range expected for the proposed structures for these complexes(Table – I). It is seen that the observed values of  the percentage of carbon,  hydrogen and nitrogen of these complexes are in close agreement with the values calculated for these complexes.

Electronic spectra  

The complex and porphyrin free bases were characterised using UV-visible  spectroscopy.  The  electronic  spectra of some complexes and  free bases  in the range 300-700nm  were recorded  and the values are  given in (Table-II).

The products [TPP and Cu(TPP)] are purple and dark red crystals, respectively19. Porphyrin macrocycles exhibit two main bands in UV-visible spectra, the soret (a more intense band around 400 nm) and the  Q-bands 500-650nm.

The appearance of Cu(TPP) and TPP differed in that Cu(TPP) was more of a dark red color and TPP was more of a vibrant purple color. The reason for this is that the intensity for TPP is much higher in the 600-700nm range (~200 a.u. at 654nm) compared to the 60a.u. at 654nm of Cu(TPP). A higher intensity in the red range results in a compound more resembling blue, which is why Cu(TPP) is redder than TPP. Metallated porphyrins such as Cu(TPP) are more symmetrical, resulting in fewer Q- bands and a simpler spectrum. The Soret Band is the highest peak in the UV-visible spectrum, around 400nm, and the Q-bands are the smaller bands in the spectrum around 500-650nm.

UV-visible spectra of Copper(II) in H2(OBTPP) which leads to formation of Cu(OBTPP) shows absorption bands at 426, 553, 586nm having a λmax value at 485nm. Cu(OCTPP) shows absorption at 448nm and 580nm. The shift is in agreement with the presence of electronegative halogen atom on these complexes as halogen are known to shift the absorption bands to longer wavelength. Cu(OCOBTPP) shows sharp peaks at 568nm, 615nm and also around 660nm with a shoulder band at 476nm. The wide variation of Cu(OCOBTPP) and its free base suggest that the complex has been formed.

The H2(OBTPP) displays  absorption bands at 372 and 466nm with well formed weak bands at 568 and 627nm.  H2(OCTPP) shows band  at 372, 408, 514, 586nm. The lmax value was observed at 469nm in the case of H2(OCOBTPP) the band were obtained at 464, 556, 598 and 644nm respectively, with lmax value at 464nm. The comparison of the spectra of the free bases show that substitution of Br atom at β-pyrrole position leads to greater shift than substitution of electronegative group in the phenyl ring.

The introduction of Cr(III) in H2(OBTPP) which leads to formation of Cr(OBTPP)Cl shows absorption bands at 384,497, 603 nm having a lmax value at 497nm. Cr(OCTPP)Cl shows  absorption at 386, 449, 565 and 643nm with a lmax value at 449 nm.

Magnetic Moment

Chromium(III) complexes show values in the range 3.79 B.M. indicating an octahedral structure (Table-I).

(Table1: Chemical Free Bases  And  Their Chromium(Iii) Complexes,  Analysis  Datas  Of  Porphyrin

S.NO.

PORPHYRIN

ANALYSIS  DATA

MAGNETIC MOMENT

μeff  / B. M.

C%

H%

N%

1.

H2(OBTPP)

42.20(42.29)

1.80(1.77)

4.28(4.49)

2.

H2(OCTPP)

59.15(59.36)

2.32(2.49)

6.24(6.28)

3.

H2(OCOBTPP)

34.32(34.71)

0.78(0.92)

3.45(3.68)

4.

Cr(OBTPP)Cl

39.40(39.65)

1.25(1.65)

4.60(4.32)

3.79

* Calculated values are given in parentheses

(Table2: Electronic  Spectral Datas Of Some Free Base Porphyrins And Metal Derivatives

S.NO.

PORPHYRIN

ABSORPTION  PEAKS ( nm)

1.

H2(OBTPP)

372, 466 (s), 568, 627

2.

H2(OCTPP)

372 (sh), 408 (s), 514, 586

3.

H2(OCOBTPP)

464 (s), 556, 598 (sh), 644

4.

H2(TPP)

420(s), 510, 545

5.

Cu(TPP)

417(s), 541, 580

6.

Cu(OBTPP)

426 (s), 553, 586

7.

Cu(OCTPP)

448 (s) , 580

8.

Cu(OCOBTPP)

476(sh), 568(s), 615,660

9.

Cr(OBTPP)Cl

384, 497 (s), 603

S=Strong,  Sh=Shoulder

References

  1. Inamo, M.; Nakajima,K.;  Bull.Chem.Soc.Japan. 1998, 71, 883
  2. Yaul,S.R.; Yaul,A.R.;  Pethe,G.B.; Aswar,A.S.;  Am.Eur.J.Sci.Res. 2009, 4,4,229.
  3. Abdou,S.; Tabi,T.; Raffat I.M.;Morsi,M.A.; Trans.Met.Chem. 2004, 29, 543
  4. Prasad, R.N.; Mathur,M.;  J.Serb.Chem.Soc. 2002, 67, 12, 825
  5. Ellis,P.E.; Lyons,J.E.;  J.Chem.Soc.Chem.Commun.1989, 1315
  6. Dolphin,D.; Felton,R.H.; Acc.Chem.Res.1974,7,26
  7. Liao,M.S.; Scheiner,S.; J.Chem.Phy.2002,117,1,205
  8. Holm,R.S.; Chem.Rev.1987.87.1401
  9. Ellis,P.E.;  Lyans,I.E.;  U.S.Patent.1997.667.328
  10. Lane,B.S.;  Burgess,K.; Chem.Rev.2003.103.2457
  11. Reddy,C.;  Hamilton,G.A.; Madystha,K.M.; Eds.Academic Press.New York.1990
  12. Sheldon,R.A.; Kochi,J.K.;  Metal catalyzed oxidation of organic compound. Academic press. New York.1981
  13. Mijs,W.J.;  De jonge,C.R.I.; Plenum Press. New York.1986
  14. Spadavecchia,J.; Ciccarella,G.; Siciliano,P.; Capone,S.; Rella,R.;  J.Mol. Catel.A. 2004, 100,88
  15. Dorough,G.D.; MillerJ.R.; Huennekens,F.M.;  J.Am.Chem.Soc. 1951,73,4315
  16. Alder,A.D.; Longo,F.R.; Vardi,V.;  Inorg.Synth. 1976, 16, 213
  17. Bhyrappa,P.; Krishnan,V.;  J.Chem.Sci. 2004, 116, 2, 71
  18. Bhyrappa,P.; Krishnan,V.;  Inorg.Chem. 1991, 30, 239
  19. Biesaga,M.; Pyrzynska,K.; Trojanowicz,M.; Talanta. 2000, 51, 209


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