ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Development and Validation of Stability Indicating Uv and Rp-Hplc Method for the Estimation of Flupritine Maleate in Bulk and Formulations

D. Gowrisankar1, N. Mallikarjuna Rao*2

1Department of Pharmaceutical Analysis and Quality Assurance, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.

2Department Of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, India

DOI : http://dx.doi.org/10.13005/ojc/300455

Article Publishing History
Article Received on :
Article Accepted on :
Article Published : 15 Nov 2014
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ABSTRACT:

The objective of the study was to develop stability indicatingUV spectroscopy and RP-HPLC method for the estimation of flupritine maleate in bulk and marketed tablets. Chromatographic separation was achieved on Agilent Eclipse C18, 150 x 4.6 mm, 5m with mobile phase consisting of Buffer(1 ml OPA and 1 ml TEA in 1000 ml water and pH was adjusted to 3 with dilute OPA) and Methanol are taken in 50:50%v/v. The effluent was monitored at 246 nm. A sharp peak was observed at 4.0 min. UV Spectrophotometric method was performed at 250 nm using methanol as the solvent. R2=0.999 for HPLC method and R2=0.998 for UV Spectrophotometric method. The method was validated as per ICH guidelines with linearity, precision, accuracy, robustness, ruggedness and specificity.Statistical analysis showed that both the methods were precise, accurate, sensitive, and Stability indicating can be used for the routine analysis of flupritine economically in bulk and commercial formulations.

KEYWORDS:

Flupritine maleate; RP-HPLC; UV-Spectrophotometric; Method development and validation; Degradation; ICH guidelines

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Gowrisankar D, Rao N. M. Development and Validation of Stability Indicating Uv and Rp-Hplc Method for the Estimation of Flupritine Maleate in Bulk and Formulations. Orient J Chem 2014;30(4).


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Gowrisankar D, Rao N. M. Development and Validation of Stability Indicating Uv and Rp-Hplc Method for the Estimation of Flupritine Maleate in Bulk and Formulations. Orient J Chem 2014;30(4). Available from: http://www.orientjchem.org/?p=5361


INTRODUCTION

Flupritine acts as a centrally acting non opioid analgesic1. Chemically it is amino pyridine derivative with the chemical name of ethyl 2-amino-6-[(4 fluorobenzyl) amino] pyridin-3-yl carbamate maleat2. Flupritine was noted for its neuroprotective properties and also investigated for use in Creutzfeldt–Jakob disease, Alzheimer’s disease, and multiple sclerosis. Literature review reveals that various analytical methods like UV Spectrophotometry3-4, HPLC5-7 have been developed However no stability indicating UV methods are reported but one stability indicating HPLC method8 was reported for the estimation of flupritine maleate Hence, an attempt was made to develop simple, accurate and precise stability indicating UV and RP-HPLC methods for the estimation of flupritine maleate in pure and their marketed formulations.

 

Figure 1 Figure 1 

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EXPERIMENTAL

MATERIALS AND METHODS

LABINDIA-3000+ UV-Visible double beam spectrophotometer with a fixed slit width 1nm and 1cm matched quartz cells was used for all the spectral measurements.Alliance WATERS e 2695 HPLC with UV, VISIBLE-2489 & PDA-2998 Detector. All reagents used were of analytical reagent (AR) grade. HPLC grade methanol and water was used throughout analysis. Flupritine sample was kindly provided by Sun pharmaceuticals Ltd.

CHROMATOGRPHIC CONDITIONS AND MOBILE PHASE IN HPLC

Chromatographic separation was achieved on Agilent Eclipse C18, 150 x 4.6 mm, 5 m. Flow rate was maintained at 1.0 ml/min with 30°c column temperature.The detection was monitored at 246 nm and the run time was 8 minutes. The volume of injection loop was 10 µl prior to injection of the drug solution. To 1000ml of HPLC grade water 1 ml of orthophosphoric acid and 1 ml of TEA were added and the pH adjusted to 3 with dilute OPA solution. The mobile contains Buffer and Methanol in the ratio of 60:40%v/v, filtered through 0.45µ membrane filter and sonicated for 20 minutes to degas. Water and Methanol(50:50) was used as diluent.

OPTIMIZED CONDITIONS IN UV

Methanol is used as diluent. 250 nm was selected as detection wavelength.

PREPARATION OF SOLUTIONS IN HPLC

Preparation of standard solution

Accurately Weighed and transferred 10 mg of flupritine maleate working Standard into a 10 ml clean dry volumetric flask, and 7 ml of HPLC grade Water was added, sonicated for 5 minutes and make up to the final volume with diluent. (Fig.2)

 

Figure 2 Figure 2

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Preparation of sample solution

20 tablets were weighed and crushed into powder, weight equivalent to 1 tablet was calculated. 5 tablets powder weight was transferred into a 200 mL volumetric flask, 150mL of diluent added and sonicated for 25 min, further the volume made up with diluent and filtered. From the filtered solution 4.0ml was pipetted out into a 100 ml volumetric flask and made up to 100ml with diluent. The % assay was found to be 100%.

PREPARATION OF SOLUTIONS IN UV

Preparation of standard solution

Accurately weigh and transfer 10 mg of flupritine Working standard into a 10 mL volumetric flask add about 7 mL of Diluent and sonicated to dissolve it completely and madethe volume up to the mark with the same solvent (Stock solution).Further pipette 0.3ml of the flupritine stock solution into a 10 ml volumetric flask and   dilute up to the mark with diluent. (Fig.3)

 

Figure 3 Figure 3

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Preparation of Sample preparation

Accurately weigh and transfer equivalent to 10 mg of Tablet powder Fpmt Working standard into a10 mL volumetric flask add about 7 mL of Diluent and sonicated to dissolve it completely and made volume up to the mark with the same solvent (Stock solution).Further pipette 0.3 ml of the flupritine stock solution into a 10 ml volumetric flask and   dilute up to the mark with diluent. The % assay was found to be 100%.

VALIDATION OF THE METHOD

Validation of the optimized method was performed according to the ICH Q2 (B) guidelines9-10.

SYSTEM SUITABILITY STUDIES

The system suitability test was carried out on freshly prepared stock solution of flupritine to check various parameters such as column efficiency, tailing factor and number of theoretical and presented in Table 1. The values obtained were demonstrated the suitability of the system for the analysis of the drug. System suitability parameter may fall within 2 % standard deviation range during routine performance of the method.

 

Table 1 Table 1

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LINEARITY

For the HPLC method for the determination of linearity, prepared standard solution at five different concentration levels. The method was found to be linear in the range of 50 to 150% of target assay concentration i.e. 200 to 600 µg/ml. peak areas were recorded and calibration curve was plotted by plotting peak areas on y axis and concentration on x axis. The calibration curve was shown in fig.3and the results are presented in table 2.In UV the method was found to be linear in the range of 50 to 175 % of target assay concentration. I.e. 10 to 35 µg/ml   measured the absorbances of the above levels at 250nm Plotted a graph of peak area versus concentration (on X-axis concentration and on Y-axis Peak area) and calculate the correlation coefficient.The calibration curve was shown in fig.4 and the results are presented in table 3.

 

Figure 3B Figure 3B

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Table 2 Table 2

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Figure 4 Figure 4

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Table 3 Table 3

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METHOD PRECISION (INTRA AND INTER DAY):

The repeatability of the analytical method under normal operational conditions was verified by injecting assay preparationssix times individually and calculated the % RSD. The same was performed on different day also. And the % RSD values for intraday and inter day precision were based on the mean and standard deviations. The % RSD was found to be less than 1% by UV and less than 2 % by HPLC, indicating that the method was sufficiently precise; the results are shown in table 4.

 

Table 4 Table 4

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ACCURACY OR RECOVERY

Accuracy is the percent of the analyte recovered by assay from a known added amount. This study is mainly useful in order to check the accuracy of the developed method and to study the interference of the formulation additives. For the measurement of accuracy data from nine determinations over three concentration levels i.e. 50%, 100% and 150 % were determined. The results are presented in table 5.

 

Table 5 Table 5

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ROBUSTNESS

Robustness of the method was verified by altering individually the chromatographic conditions like mobile phase composition (10%) , flow rate (±2 nm), column temperature (±5°C), etc.it was observed that no much variation in system suitability parameters even after these small delibral changes.These results proved that the developed method was robust.In UV method the wave length was varied at 248 nm to 252nm. On evaluation of the above results, it can be concluded that the variation in wave length affected the method significantly. Hence it indicates that the method is robust even by change in the wave length.

FORCED DEGRADATION STUDIES

The International Conference on Harmonization (ICH) guideline entitled stability testing of new drug substances and products requires that stress testing be carried out to elucidate the inherent stability characteristics of the active substance. The aim of this work was to perform the stress degradation studies on theflupritine using the proposed methodForced degradation of the flupritine maleate drug substance was performed under acid, alkaline, peroxide, thermal, photolytic and hydrolysis stress conditions. The procedure and results were summarized in the table 6& 7.

TABLE 6: SUMMARY OF DEGRADATION STUDIES BYHPLC

Mode of degradation

 

Conditions

 

Assay (mg/tablet)

 

FLUPRITINE MALEATE

%Degradation w.r.t. control

Purity

angle

Purity

Threshold

Control

No treatment

398.2

Acid

degradation 5N  HCl

40°C/5min

369.2

7.30

0.131

0.289

Alkali degradation 1N NaOH

80°C/1hr

360.76

9.40

0.153

0.293

Peroxide degradation 30%W/V H2O2

80°C/10min

293.76

26.20

0.209

0.296

Thermal degradation

105°C/72hrs

376.32

5.50

0.195

0.306

Photolytic degradation

UV/72hrs

331.56

16.70

0.176

0.304

Humidity degradation

25°C/90%RH/

72 hrs

395.08

0.80

0.146

0.289

 

Table 7 Table 7

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RESULTS AND DISCUSSION

The developed methods for estimation of flupritine maleate were found to be accurate, simple and precise. The simplicity and ease of the developed methods lie in using Methanol as solvent.

The linearity of both HPLC and UV methods showed excellent correlation coefficient of 0.999.

In precision studythe% RSD was found to be less than 1% by UV and less than 2 % by HPLC, indicating that the method was sufficiently precise. Both methods showed good recovery and the recovery values are found between 98 to 101 % and % RSD was found to be less than 1% indicating accuracy of the method. The method was found to be robust because system suitability was passed even though deliberal changes were made in the developed HPLC and UV methods. In degradation studies it was observed that purity threshold value greater than the purity angle in each condition value it indicates the noninterference of the excipients, degradants with the analyte peak.

CONCLUSION

The developed methods are able to analyze the commercial formulations economically.

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