Extractive Spectrophotometric Determination of Levetiracetam in Pharmaceutical Formulations

K. Srinivasu¹, J. Venkateswara Rao^2*, N. Appala Raju² and K. Vanitha Prakash³

¹Dr.Reddys Laboratories Ltd. ARD, Hyderabad (India).

²Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Hyderabad- 500 034 (India).

³Institute of Science and Technology, JNTU, Hyderabad (India).

ABSTRACT:

Three simple, economical, precise, reliable and reproducible visible spectrophotometric methods (A, B and C) have been developed for the estimation of Levetiracetam in bulk as well as in Tablet formulation. The developed methods A, B and C are based on the formation of chloroform extractable complex of Levetiracetam with Bromocresol green (method A), Bromophenol blue (method B) and Bromothymol blue (method C) which shows absorbance maxima at 435 nm, 454 nm and 415 nm respectively. The absorbance-concentration plot is linear over the range of 2.5-25 mcg/ml for Method A, 2.5-25mcg/ml for Method B and 1.5-15 mcg/ml for Method C. The different experimental parameters affecting the development and stability were studied carefully and optimized. Results of analysis for all the methods were validated statistically and by recovery studies.

KEYWORDS:

Levetiracetam; Bromocresol green; Bromophenol blue; UV double beam spectrophotometer

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Copy the following to cite this article: Srinivasu K, Rao J. V, Raju N. A, Prakash K. V. Extractive Spectrophotometric Determination of Levetiracetam in Pharmaceutical Formulations. Orient J Chem 2008;24(3).

Copy the following to cite this URL: Srinivasu K, Rao J. V, Raju N. A, Prakash K. V. Extractive Spectrophotometric Determination of Levetiracetam in Pharmaceutical Formulations. Orient J Chem 2008;24(3). Available from: http://www.orientjchem.org/?p=25043