Characterization, Biological Activity and DNA Studies of Atomexetine and Ortho hydroxy benzaldehyde Imine Metal Complexes

The rapid increasing applications and anti-bacterial and anti hyperactive properties of different drug the author have synthesized Imine complexes of Atomexetine with O-hydroxy benzaldehyde. These were depicted with various techniques via Elemental Analysis, UV, FT-IR, NMR, ESR, VSM, Conductivity and TG-DTA. These ligands and metal complexes were also screened for biological activity and DNA Studies.


INTRODUCTION
Imines are the condensed products of Carbonyl compounds and Amines 1 . These are also known as Schiff Bases. The Scientist Hugo Schiff was introduced in 1864 2 . Imines show a unique property in the participation of covalent bond with transition metal ions, since the easy formation of ligands act as intermediates for the formation of complexes with transition metal ions. Imine complexes perform biological activities like anti inflammatory, anti fungal, anti bacterial, anti viral, anti diabetic, anti cancer and in pharmaceuticals etc [3][4][5][6][7][8][9] . Biological activity of the Ligands were recorded by the presence of >C=N moiety of the Schiff bases. These are also having uncountable appliances in technical fields like automobiles, electro plating, printing technology, textile and detergents [10][11] .
The author has reported characterization, biological activity and DNA studies of the ligands and their metal complexes. The synthesis of Imine metal complexes has been carried out by conventional method and characterization with various techniques like FT-IR, H 1 -NMR, ESR, UV-Vis and Conductometry. Thermal stability of the complexes was identified by TG-DTA with various temperatures. Biological activity of Imines and their Cu and Ru complexes was performed in In vitro conditions and DNA binding mode with UV-Vis Spectroscopic technique.

Instrumentation
IR Spectral data with KBr Pellets, H 1 -NMR Spectral data with BRUKER 400MHZ SUPERCON Spectrometer, ESR Spectral data with Bruker ESP 300E spectrometer, UV-Vis Spectral data with Schimadzu UV-1800 model Spectrometer, XRD on Panalytical X'pert3 difractometer, conductivity measurements with digital conductivity meter DCM-900, VSM with EG and G-155 magnetometer were recorded.

Synthesis of OHB-AT Ligand
The equal concentration of AT and OHB was dissolved separately, this mixture was heated for 2 h by adding few drops of concentrated HCL, pink colored solution was obtained and this was cooled to room temperature. Then pink color precipitate was obtained. These crystals were washed with methanol and dried in micro wave. The percentage yield of ligand was 80.
conditions with different micro-organisms and DNA studies on UV-Vis Spectrophotometer.

IR spectral data
The nature of Imine metal complexes were identified by obtaining strong band at 1627 cm -1 indicated the development of Imine group, which on complexing with the metals viz. Cu (II) and Ru (III) the bands appeared at 1605 cm -1 and 1622 cm -1 indicated the coordination between Imine group of nitrogen with electron deficient metal ions because of decreased electron density on Nitrogen atom 12,13 . The another strong bands at 420 cm -1 and 429 cm -1 for copper and ruthenium expressed coordination between metal and ligand 14,15 . IR data of the ligand and complexes were represented in the Table 1.

Synthesis of OHBAT-Cu(II) & Ru(III) metal complexes
The complexes were prepared by mixing 1:2 ratio of an aqueous solution of metal ions with the methanolic solution of Imine separately and stirred with magnetic stirrer for proper mixing of contents. Then the contents in the flask were reflexed for six hours and kept in ice bath for cooling, bluish green color sharp needle like crystals were obtained. These were washed and recrystallized with methanol. The yield of copper and ruthenium complexes was 75% and 78%.

RESULTS AND DISCUSSIONS
The main purpose of the present work is to study the characterization of metal complexes with various techniques, Biological activity in In vitro NMR spectral data A singlet at 5.6 ppm revealed aromatic-OH protons of OHB, this was absent in complex given the coordination between oxygen moiety of OHB and metal ions of Cu (II) and Ru (III). A singl et at., 6.26 ppm specified the presence of Imine group of ligand 16 , this was shifted to 7.6 ppm and 7.26 ppm by the coordination of ligand with metal ions. Another singlet at 4.1 ppm and 4.65 ppm appeared only by the coordination of water molecules with metal ions. NMR data of the ligand and complexes were represented in the Table 2.

UV-Vis spectral data
The transition of ligand was 277 nm, which on complexation with Cu (II) and Ru (III) metal complexes change in transitions at 289 nm and 306 nm signified the charge transfer transition from L→M 18 . UV-Vis Spectral data was represented in the Table 4.

Analysis of TG-DTA
By observing modifications at different temperature ranges one can easily explain thermal stability of the complexes. The temp ranges between 110 0 C-222.10 0 C and 215 0 C-280 0 C specified the loss of two water molecules at the first level 20 , the second level at 261.03 0 C and 450 0 C specified the formation of stable intermediate peaks as the results of decomposition at the temperature range of 818 0 C and 600 0 C. At high temp metallic oxides formation takes by the exothermic process. Thermal data of Cu (II) and Ru (III) metal complexes were represented in the Table 7.

Conductometry
Molar Conductivity value of the complexes at 58 and 60 Ohm -1 cm 2 mol -1 for Cu(II) and Ru(III) specified non electrolytic in nature 21 . Molar conductance values of the complexes were represented in the Table 8.

VSM analysis
The magnetic momentum values of OHBAT-Cu and OHBAT-Ru complexes at 4.28 and 5.56 BM specified octahedral geometry 22,23 of the complexes due to the presence of lone pair of electrons in d-orbital of the metal ions. The magnetic susceptibility values of OHBAT-Cu and OHBAT-Ru complexes were represented in the Table 9.

Biological studies
Biological studies of the ligands and complexes were performed in In vitro conditions; represented biological activity of Imines was less than their corresponding metal complexes because of the reduced electron density of metal ions by the transfer of charge, according to Chelating theory 24 . The biological activity values of Imines and their metal complexes were represented in the Table 10.  14 16 DNA binding mode of the complexes DNA binding activity of the complexes was performed with di sodium salt of calf DNA. The spectrum was performed in the absence and presence of CT-DNA. In the presence of CT-DNA the complexes conveyed hypochromic shift because of the presence of chromophores of the ligand. The quantitative comparisons of binding parameters from the following equation The binding constants of the complexes were represented in the Table 11.

CONCLUSION
In the present article author has reported characterization, biological activity and DNA studies of the metal complexes of Cu (II) and Ru (III) metal ions. The characterization reports suggested, the complex was mono nuclear with molecular formula of [M (L) 2 ]. The stoichiometry of the complex was 1:2 ratios and proposed geometry of the complexes was octahedral. The complexes were exhibit more biological activity than their corresponding ligands.

1)
I would like to express my deepest appreciation to my Professor J. Sreeramulu garu department of chemistry, SK University, Anantapuramu for his continuous support in my research area.
2) I would like thanks to RGM college of Engineering and technology for encouraging in the area of research.