Synthesis and Biological Activity of Novel ( E )-N ’-( Substituted )-3 , 4 , 5-trimethoxybenzohydrazide Analogs

The hydrazide-hydrazone analogs 4a-4l is described via the condensation of 3,4,5trimethoxybenzohydrazide 3 with various aromatic and hetero aromatic aldehydes a-l. Various spectroscopic techniques viz., (1H NMR, 13C NMR, IR and MS) were utilized to determine the structures of synthesized compounds. These compounds were evaluated for antibacterial, antifungal screening against S.aureus, S.pyogenes, E.coli, P.aeruginosa, Aspergillus niger and Candida albicans (Fungal strains). The results revealed that most of the hydrazone derivatives exhibited significant antibacterial activity. Furthermore, the synthesized hydrazone derivatives were found to exhibit significant antidiabetic activity when compared to insulin.


INTRODUCTION
Eudesmic acid is a o-methylated analogue of gallic acid and is found in Eucalpytus spp 1 .The chemical name of gallic acid, is 3,4,5trihydroxybenzoic acid, and exists either in the pure form or as a phenoilic constituent in various parts of the plant morphology such as roots, leaves, bark and fruits 2. 3,4,5-trihydroxybenzoic acid possess analgesic activity 3 ,anti-inflammatory 4 ,hepatoprotective 5 , antitumor potentials 6 , antimutagenic 7 , neuroprotective activity 8 , and toxicity against cancer cells 9 .3,4,5trihydoxybenzoic acid (gallic acid) and its associated analogs are used in the following applications such as (a) photographic developer, (b) testing of free mineral acids, (c) di-hydroxy acetone and alkaloids 10 .Furthermore, in cases of internal hemorrhoids these are known as (d) a styptic agent (e) an astringen.

Biological evaluation Antimicrobial activity
The zone of inhibition (ZI) values of hydrazone derivatives 4a-l is complied in Table 1.The results revealed that, compounds 4a-f showed excellent antimicrobial activity with ZI (15-21 mm) whereas compounds 4g, 4h, 4i and 4l showed good activity with ZI (12-15 mm) and the remaining two compounds 4j and 4k displayed moderate activity with ZI (8-10 mm) against S.aureus and S.pyogenes.Compounds 4j and 4k displayed moderate activity with ZI (10-12 mm), compounds, 4g-i and 4l showed good activity with ZI (11-14 mm) while the compounds 4a, 4b, 4c, 4d, 4e, and 4f exhibited excellent activity with ZI (17-20 mm) against E.coli and P.aeruginosa.A careful observation from the ZI values indicated that within the series of hydrazones derivatives compounds 4f, 4b and 4d showed highest ZI values compared to the standard drug ampicillin (ZI = 14-16 mm).In case of Aspergillus niger (MTCC 282) and Candida albicans (MTCC 227), compounds 4a-f showed moderate antifungal activity with ZI (10-14 mm) when compared to the standard Greseofulvin (ZI = 22 -25 mm).It's noteworthy to mention that hydrazone derivatives 4a-l showed significant antibacterial inhibition in comparison to the fungal activity.

Antidiabetic activity
Experimental in vivo studies of alloxan induced diabetic model in rat was carried for the all the synthesized hydrazone derivatives in order to understand the anti diabetic property.The results of the measurement of blood glucose (%) level are presented in Table 2. Though there is a certain varied difference in the blood glucose levels within the treatment of diabetic rats, it is evident from the table-2 that most of the hydrazone compounds exhibited significant anti diabetic property (a significant reduction in blood glucose) compared to control diabetic rats at 50 mg/kg body weight for 3 rd and 7th days.
For the antidiabetic study, insulin was taken as the standard drug at the dose of 50 mg/kg.p.o which showed 69.77% blood glucose lowering activity.Within the series of the synthesized hydrazone derivatives, compound 4d (67.95%) having R = 3-hydroxy-5-(hydroxymethyl)-2-methylpyridin exhibited significant antidiabetic activity when compared to insulin (50 mg/kg b.w) in dropping the blood glucose level.It is interesting to deduce that compounds 4f (66.0%) having R = imidazo[1,2-a]pyrimidin-2-yl)methylene, 4e (65.66%) having R = 1H-indol-3-yl)methylene, 4a (64.83%) having R = quinolin-4-yl)methylene) and 4b (63.62%) having R = 4-phenyl-1H-imidazol-2-yl) methyl displayed an substantial antidiabetic activity (increase in hypoglycemic activity).Compound 4c (58.06%) and compound 4i (58.06%) relatively had shown moderate hypoglycemic activity whereas the compounds 4l, 4j, 4g, 4h, and 4k show 55.80%, 55.68%, 55.15, 54.80 and 54.57% poor hypoglycemic property respectively.In general, it is significant to observe that hydrazone derivatives embedded with nitrogen-containing heterocycles exhibited vital hypoglycemic activity and the rest of the compounds in the series displayed modest to poor hypoglycemic activity.From the ascertained result, it is concluded that compounds 4a, 4b, 4d, 4e, and 4f reduced the glucose level in diabetic rats.But the impact of 4d is more distinct in alloxan induced diabetic rats.Investigation into additional toxicity studies and understanding the mechanism of action of hypoglycemic activity will offer an efficient drug candidate for diabetes mellitus.

MATERIALS AND METHODS
All the chemical and solvents used for the synthesis were analytical standard from Fluka or Merck.For thin-layer chromatography (TLC) analysis, E.Merck AL silica gel 60 F254 plates were utilized and spots were visualized under UV light.The mass spectra was recorded on Agilent ion trap MS and Infrared (IR) spectra were recorded on a Perkin Elmer FT-IR spectrometer. 1 H NMR and 13 C NMR spectra were recorded in DMSO-d 6 with a 400 MHz (Varian Mercury plus) instrument.TMS was used as an internal standard and the chemical shift values were reported in ´ (ppm) and the signals were reported as s (singlet), d (doublet), dd (doublet of doublet), t (triplet), q (quartet), m (multiplet) and coupling constants are measured in Hz.Melting point (mp) determinations were performed by using Mel-temp apparatus and are uncorrected.The aldehydes f and l 21,22 was synthesized as per the previous reported literature procedures.

3, 4, 5-trimethoxybenzohydrazide 3 M e t h o d 1 :
A m i x t u r e o f 3 , 4 , 5-Trimethoxybenzoic acid 2 (1 g, 4.71 mmol) and Amberlyst -15 (300 mg) in methanol (20 mL) was refluxed for 24 h in a pressure tube.T.L.C of the reaction indicated absence of starting material; the insoluble Amberlyst-15 was filtered at the pump and rinsed with methanol (4 X 2 mL).The combined methanol filtrates (containing methyl 3, 4, 5-trimethoxybenzoate) was further reacted with hydrazine hydrate (1.09 mL, 21.8 mmol) and heated at its reflux temperature for 10 h.The reaction mixture was concentrated to one-fourth volume and cooled to 5 o C to isolate compound 4 (3, 4, 5-trimethoxybenzohydrazide) as a white solid.Yield: 74%; M.p.: 156-158°C; 1  Method 2: Compound 2 (1 g, 4.71 mmol) was dissolved in THF (10 mL) containing DIPEA (5.65 mmol) and cooled to 5°C.To the above solution, HATU (1.79 g, 4.71 mmol) was added in five portions and stirred at room temperature for 4 h.Water (20 mL) was added to the reaction contents and was taken in dichloromethane (50 mL), the organic layer was separated, washed with water (2 X 15 mL) and then with brine solution.The dichloromethane layer was dried over Na 2 SO 4 , filtered and concentrated under reduced pressure to isolate compound 3. Yield: 95%.

Preparation of Hydrazone derivatives 4a-l
Aromatic/heteroaromatic aldehydes a-l (1.0 mmol) was added to ethanol (2 mL) containing compound 3 (100 mg, 0.40 mmol) and heated to reflux point for 1h.The solids obtained was filtered and slurred with ethanol (1mL) followed by n-Hexane to get the hydrazones 4a-l .

Biology Antimicrobial activity test
The antibacterial and antifungal screening test of hydrazones 4a-l was done as per the standard agar diffusion method 23 .The microbial strains utilized for the study are (a) Aspergillus niger (MTCC 282), Candida albicans (MTCC 227) categorized under fungal strain (b) Escherichia coli (MTCC 443), Pseudomonas auriginosa (MTCC 424), Staphylococcus aureus (MTCC 96), Streptococcus pyogenes (MTCC 442) categorized under bacterial strains.Ampicillin(for antibacterial study) and Greseofulvin (for fungal study) are used as the standard reference drugs respectively under alike conditions for assessment.1% Dimethyl sulphoxide was used as a blank reading.The MIC (minimum inhibitory concentration) was found at 50 µg/mL (using dimethyl sulfoxide as solvent) for the both fungal and bacterial study.The culture strains of fungi was maintained on potato dextrose agar (PDA) slant at 27±0.2°C for 24-48 hrs (till sporulation) while the bacteria was maintained on nutrient agar slant at 37±0.5°C for 24 h.The antimicrobial activity was assessed as follows (i) in case of antibacterial study: nutrient agar plate seeded with 0.1 mL of the respective bacterial culture strain suspension prepared in sterile saline (0.85%) of 105 CFU/mL dilutions (ii) in case of antifungal study: Sterile PDA plate was prepared containing 2% agar; 0.1 mL of each fungal spore suspension was spread on each plate and incubated at 27±0.2 °C for 12 hrs.Target compounds viz., synthesized hydrazone derivatives (0.1 mL), dilution ranging from 25 to 1000 ¼g/mL was poured into wells (6 mm diameter) separately for individual bacteria and fungi strains.All the plates were incubated at 37±0.5°C for 24 h (for bacterial study) and 27±0.2 °C for 12 hrs (for fungal study) and respective MIC values were determined for all the compounds.By measuring the diameter of the inhibition zone around the well (in mm, including the well diameter), the zone of inhibition was calculated.The readings were recorded three times and the average values were tabulated (data are expressed in the form of mean of inhibition zone diameter for test compound performed in triplicate ± SD).

Pharmacological evaluation Acute toxicity studies
Assembly of six mice (albino, weighing ~20-25 g) were fasted all night and administered orally with the test compounds 24,25 .The dose of the compounds was assorted from 500 mg kg "1 body weight.Signs of acute toxicity viz., motor activity, lacrimation, sedation, tremors, convulsion was observed for the mice for 24 h and the test results revealed that no mortality of the mice was observed even beyond 24 h.Based on the above observation, the test compound's cut-off value, LD 50 was fixed as 50 mg kg "1 , consequently that 500 mg kg "1 i.e., 1/10 of cut-off value was taken as the dose quantity for assessment of antidiabetic activity.Animal experiments involved during the course of the study were undertaken by the approval of (a) Institutional Animal Ethics Committee, Anurag Group of Institutions (formerly Lalitha college of Pharmacy), Hyderabad, India and also (b) guidelines of CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), IAEC ( Institutional Animals Ethics Committee) followed for the protection of animals.IAEC No:I/IAEC/ LCP041/2014/SM40.

Antidiabetic activity 26, 27 Alloxan induction of experimental diabetes
Overnight-fasted swiss albino mice are injected with alloxan (50mg/kg) in saline buffer intraperitoneally.After 72 hours, blood glucose levels were determined and mice having blood glucose levels <145mg/dl were excluded from experiment and rest were divided into ten groups.After 72 h of alloxan injection, confirmation of hyperglycemia was observed in animals.

Protocol for Animal Experiments
Animals were separated into ten sets of six mice (n = 6): (i) diabetic animals (vehicle) labeled as set-1, received 1m L of CMC (0.5%); (ii) diabetic animals labeled as set-2, received insulin 50 mg/Kg.
(iii) Set (3-10) diabetic animals received compounds 4a-4l in a single dose (50 mg/kg body weight per oral) respectively for seven days incessantly

Measurement of Blood glucose levels
Each individual reading was noted by withdrawing blood sample from the tail vain.Measurement of blood glucose was taken at intervals of 0, 3 rd and 7 th day.By snipping the tip of the tail, blood samples were withdrawn from a tail vein and the blood glucose level was measured at the end of 0, 3 rd and 7 th day (by Accu Sure Blood Glucose Monitoring System, Dr. Gene Health & Wellness).

Computational analysis
The blood glucose level readings are presented as mean ± SEM and were determined using analysis of variance and group means were differentiated with Tukey-Kramer Post ANOVA test.The readings were considered when P < 0.01.

CONCLUSION
The synthesis of some novel (E)-N'-(substituted)-3,4,5-trimethoxybenzohydrazide derivatives is described from commercially available gallic acid.The newly synthesized hydrazone derivatives 4a-4l was evaluated for antimicrobial and antidiabetic activity.In case of bacterial and fungal studies, compounds 4a, 4b, 4c, 4d, 4e, and 4f exhibited excellent antibacterial activity and moderate antifungal activity.Hydrazone derivatives embedded with nitrogen-containing heterocycles exhibited vital hypoglycemic activity and the hydrazone compounds embedded with aromatic ring displayed modest to poor hypoglycemic activity.