Silver Nanoparticle Loaded Activated Carbon : An Escalated Nanocomposite with Antimicrobial Property

In this work, the antimicrobial property of nanocomposite prepared by loading of silver nanoparticle onto activated carbon (AgNP-AC) was studied. The silver nanoparticles were synthesized biologically from the culture Enterobacter aerogenes and loaded onto the activated carbon. The nanoparticle and nanocomposite were characterized by UV-vis, scanning electron microscopy and X-ray diffraction studies. The spectroscopic assay and zone inhibition test for E. coli and B. subtilis clearly illustrates the antibacterial activity of the silver nanoparticle and nanocomposite. The antimicrobial activity of the nanocomposite was of the nanocomposite was also confirmed with the observed zone of inhibition against beverage, brewery, dairy and dye industrial effluents.

Many methods have been adopted for treatment of waste water and industry effluents.One of the best and convenient water treatment process is by adsorption, as it is cheap, easy and simple in operation 1 .The most popular and commonly used adsorbent in water and wastewater treatment throughout the world is activated carbon (AC) 2 .AC as adsorbent have relatively high surface area, large porosity, total pore volume and presence of wide spectrum of functional groups on its surface, which provides a strong affinity for even low concentration organics to attach to itself 3 .The well developed internal pore structure of AC with macropores serves as excellent loci for colonization of organisms and support material for bacterial growth 4 .The biofilm layer formations on the AC by the microbes have undesirable effects: the filter may clog up as a result of excessive bacterial growth.Nowadays, nanoparticles are used as sorbents for organic and inorganic pollutants removal because of their high specific surface area and also a large number of unsaturated atoms on their surfaces that can bind readily with most of other atoms 5,6,7 .More attention is focused to water treatment with engineered nanoparticles 8 .These nanoparticles can be produced by numerous techniques, including chemical, aerosol, electrochemical, laser irradiation, sonochemical deposition, photochemical reduction and biological techniques 9,27 .The biotechnological experimental processes gains importance because of the advantages like safe, cost-effective, sustainable and environmentally friendly processes 10 .The nanoparticles has very attractive properties such as an ordered structure with a high aspect ratio, ultralight weight, high mechanical strength, electrical and thermal conductivity and high specific surface area.The nanoparticles property differs from those of their corresponding bulk state 11 .Among the nanoparticles, silver nanoparticles (AgNP) are gaining more importance because of its antimicrobial and antiviral properties 12 .The believed mechanism is that AgNPs attach to the surface of cell membrane disturbing the permeability and respiration functions of the cells, which leads to microbial cell death 13 .Nanocomposite as an adsorbent will prove as an efficient adsorbent because of the increase in their surface-to-volume ratio with the reduction of the size of the adsorbent particle from bulk to nano dimensions 14 .In the present study, silver nanoparticles were biogenically synthesized using Enterobacter aerogenes and the synthesized nanoparticles were incorporated onto activated carbon to produce a nanocomposite.These nanoparticles and nanocomposites were characterized using UV-vis spectroscopy, SEM and XRD.Further the antimicrobial activity of silver nanoparticles and nanocomposites were studied against E. Coli, B. Subtilis and microbes present in the various industrial effluents like beverage, brewery, dairy and dye respectively.

MATERIALS ANd METHOdS
All chemicals used in the analysis are of analytical grade and were procured from Sigma Aldrich & Hi Pure chemicals, India and did not require any further purification.The culture Enterobacter aerogenes (MTCC 111) was obtained from Microbial Type Culture Center (MTCC), Chandigarh, India.

Synthesis of AgNP
In 100 ml of 1mM silver nitrate (AgNO 3 ) solution, 5 % (v/v) of Enterobacter species culture supernatant sample was added slowly and was observed for colour change.The particles resulting from the reduction of AgNO 3 are called as AgNP.

Preparation of Nanocomposite
The AgNP was loaded on AC by means of simple mixing.1.5 g of AC was taken and added to result AgNPs solution.It was mixed vigorously by continuous stirring for 2 h at 150 rpm using Meditek Orbital rotary double deck shaker and Ultrasound radiating the solution for 40 min at 100 Hz using Ultrasonic Processor P2 (Vibronics).The nanocomposite product was obtained by drying the AgNP loaded activated carbon (AgNP-AC) powder in air at a temperature of 75 °C.

Characterization studies
The Surface plasmon resonance (SPR) property of the synthesized AgNP was measured using Systronics UV-vis double beam spectrophotometer 2202.The morphological, size and elemental characterization of the normal activated carbon and the AgNP-AC was carried out using a FEI Quanta FEG 200 FESEM at an operating voltage of 10kV.The XRD pattern of the AC and Ag-NP was determined using Spectris Technologies X'pert Powder X-ray diffractometer with Cu Ká radiation(40 kV and 30mA) for 2è values over 4-100°C.

Antimicrobial studies AgNP -direct method
The antibacterial property of AgNP was determined using zone inhibition test and indirect growth curve method (cell number versus time of incubation) for E. coli and B. subtilis.For zone inhibition test the 100 µl of the organisms E. coli and B. subtilis grown overnight in Luria broth (LB) medium was spread on a nutrient agar plate.The plates were then punctured and fed with a colloidal AgNP solution and incubated at 37°C.The zone of inhibition was then measured after 24 h of incubation.

AgNP -Indirect method
LB media was prepared in a sterile manner and 100 ml of the media was poured into flasks labeled as A, B, C &D.A 100 ml of E. coli and B. subtilis bacterial suspension was inoculated into flasks A, B & C, D respectively. 1 ml of the resultant AgNP from 5mM AgNO 3 solution was added into flasks B and D. Flasks A & C was kept as control, while B & D as the test for E. coli and B. subtilis, accordingly.The flasks are incubated in a rotary shaker at 37°C.The growth was monitored for the control and the test by withdrawing aliquots of control and test samples for every 20 min and measuring the absorbance at 600 nm using a Mapada V-1100 D spectrophotometer.A graph was plotted for time in min (abscissa) Versus OD at 600 nm (ordinate).

Nanocomposite
T h e a n t i b a c t e r i a l p r o p e r t y o f t h e nanocomposite was evaluated using zone inhibition method.A 100 ml of E. coli and B. subtilis bacterial suspension cultured in LB medium was spread on a nutrient agar plate.The plates were then punctured and loaded with normal activated carbon (control) and AgNP-AC (Test) to find the antibacterial properties; the plates were then incubated at 37°C.The zones of inhibition were measured after 24 h.

Industrial Effluents
T h e a n t i b a c t e r i a l p r o p e r t y o f t h e nanocomposite was evaluated using zone inhibition method.A 250 ml of beverage, brewery, dairy and dye industrial effluents were spread on a nutrient agar plate.The plates were then punctured and loaded with normal activated carbon (control) and AgNP-AC (Test) to find the antimicrobial properties; the plates were then incubated at 37°C.The zones of inhibition were measured after 24 h.

Extracellular production of AgNPs
The AgNO 3 solution was reduced to AgNPs due to the addition of the supernatant containing extracellular material produced by Enterobacter species.Fig. 1 shows the change of colour from colourless to yellow-brownish, which clearly indicates the formation of AgNPs in the reaction mixture.The characteristics colour of the colloidal silver solution is due to the excitation of surface plasmon vibrations in the nanoparticle and provides a convenient spectroscopic signature of their formation.The principle behind this process is the activity of a most abundantly found enzyme nitrate reductase which reduces nitrate to nitrite and thereby reducing Ag + ions to AgNPs.Several hydroquinones with excellent redox properties were reported that could act as electron shuttle in metal reductions.Thus, it was evident that electron shuttle or other reducing agents released by Enterobacter species are capable of reducing silver ions to AgNPs 15,16,17

SPR of AgNP
The AgNPs were characterized using UV-visible spectroscopy , the results is shown in Fig. 2. A strong, broad peak located in 410-420 nm was observed for the AgNPs prepared using the Enterobacter species.Observation of this peak assigned to a surface plasmon is a strong evident for the formation of silver metal nanoparticles 18,19,20 .

Morphological Characterization Silver Nanoparticle
Fig. 3(a) shows the SEM micrograph recorded for AgNP range from 25-85 nm.The morphology of the nanoparticles was found to be spherical, monodispersed and uniformly distributed.The particles were not aggregated, which might be an indication for the presence of caping agent.The SEM images for AC and AgNP-AC were shown in Fig. 3 (b) &3 (c) respectively.AC was in the range of 125-185 nm and AgNP in nanocomposites were recognized as bright dots, which confirms the incorporation process was successful.

XRd Pattern
The XRD patterns of AC and AgNP-AC are shown in Fig. 4. The broad peaks of AC and the sharp peaks of silver appeared in the results of the AgNP-AC display the variations occur in both AgNP-AC and AC.The XRD pattern of AC did not show any diffraction peak indicating that it is disordered (Fig. 4A) 5 .As shown in Fig. 4 (B), the pattern of AgNP-AC exhibits peaks at peaks at 2q angles of 38.15°, 46.5°, 64.5° which corresponds to AgNP 21 .

Antimicrobial property Study Silver nanoparticle -direct method
Antibacterial test by zone of inhibition was done to qualitatively determine the level of inhibition using the synthesized colloidal AgNP.Fig. 5 shows the antibacterial zone of AgNP for E. coli (Fig. 5a) and B. subtilis (Fig. 5b).For both the organisms the zone of inhibition was 1.7cm, which correlates with the antibacterial property of the synthesized AgNP In both the cases the control (A): only activated carbon did not show any inhibition, but the test (B): AgNP-AC significantly showed zone of inhibition of 1.8 and 1.7 cm for E. coli and B. subtilis respectively.The zone of inhibition of the test shows that the nanoparticles of silver were well incorporated onto the activated carbon and thereby responsible for the antibacterial activity 25 .The nanoparticles introduction to the activated carbon also decreases the pore volumes and surface area 26 .We suggest that the nanoparticle incorporated activated carbon can be used as an efficient adsorbent instead of normal activated carbon, as the nanoparticles incorporated onto activated carbon will influence the growth of

Antimicrobial Test for Industrial Effluents
Antibacterial test by zone of inhibition was done to qualitatively determine the level of inhibition using the synthesized AgNP-AC nanocomposite for the four effluents were shown in Table 1.For all the industrial effluents an appreciable level of zone of inhibition was observed, the zone of inhibition was 18 mm, 20 mm, 16 mm and 18 mm for brewery, dairy, beverage and dye effluents accordingly.The results clearly shows that the AgNP loaded activated carbon nanocomposite possess the antibacterial activity.This property of the prepared nanocomposite adsorbent will prevent the bacterial biofilm formation over the adsorbent and increases the possible reuse of the adsorbent in the waste water treatment processes, without loss in adsorption property.

CONCLUSION
Silver nanoparticles were synthesized successfully by a simple biogenic route.The synthesized nanoparticles were characterized using UV-vis spectroscopy, SEM and XRD.The incorporation of synthesized AgNP onto activated carbon was done and the nanocomposite was prepared and confirmed.The growth curve estimation method for E. coli and B. subtilis shows that the synthesized AgNP inhibits the microbial growth proving its antibacterial property.Zone inhibition test showed that the AgNP-AC is possessing antibacterial activity to that of normal activated carbon, which provides the possible use in waste water treatment process preventing the biofilm layer growth over the adsorbent.Finally, silver nanoparticle loaded activated carbon will be a desirable adsorbent in water treatment process.

Fig. 1 :Fig. 2 :
Fig. 1: Colour change of silver nitrate 1mM solution from colourless to yellow brownish as the reaction proceeds on addition of the culture supernatant