A Study of the Fatty Acid Profile in the Muscle of Monopterus chuchia

The nutritional capacity of the fish Monopterus chuchia in terms of its fatty acid content was investigated to get an insight of their fatty acid composition in their most consumed part i.e. their muscle tissue. Our investigation shows the presence of SFA’s like caproic acid, caprylic acid, palmitic acid, pentadecanoic acid, heptadecanoic acid, behenic acid, MUFA’s like myristoleic acid, palmitoleic acid, oleic acid and nutritionally important PUFA’s like Eicosapentaenoic acid, docosahexaenoic acid in major amount. Erucic acid, which is reported to be an anti-nutritional factor, is present in extremely low concentration. Thus the fish which is generally consumed by very few can be farmed as a cheap nutritional source for the masses.


INTRODUCTION
It is a well known fact that besides being a beneficial protein source, fishes are a source of polyunsaturated fatty acids (PUFA).Fatty acids are essential nutrients in humans [1][2][3][4][5] and other animal diet.Compared to chicken and beef the content of n-3 PUFA is higher in fish.These n-3 PUFA's have cardioprotective 6 , anti atherosclerotic 7 , antithrombotic 8 properties.These poly unsaturated fatty acids can also prevent arrhythmias, endothelial dysfunction, high blood pressure, sudden strokes and heart attack [9][10][11] .
According to an article in Circulation 12 as fatty acids in fish block dangerous heart rhythms, eating oily fish at least twice a week may be helpful in preventing sudden death from heart attack.Excessive sodium and calcium currents in the heart which can cause dangerous unpredictable changes of individual heart cells show that fatty acids are capable of blocking such currents.It is also seen that a diet rich in oily fish is capable of reducing the effects of arthiritis 14 by producing an antiinflammatory fat.According to French researchers people who eat fish at least once a week are less prone to dementia 13 .Some researchers of Edinburg and Stirling Universities even suggest that fish could be of help in dealing with childhood autism.Thus the importance of consumption of unsaturated fatty acids, the best source of which is fish oil cannot be ignored.So effort to detect and preserve both fresh water and salt water fish rich in unsaturated fatty acids is evident throughout the world [15][16][17][18] .
Monopterus chuchia is a fish very common in West Bengal 19 inhabiting fresh and brackish waters.It is found at an altitudinal range (76 -1350) m.This fish belongs to the family Synbranchiformes.They have a burrowing habit 20 .It can thrive in various adverse conditions such as high temperature, shallow water and low oxygen levels.In West Bengal and Bangladesh the fish is consumed by tribal people and people of few other castes.It is prepared as soup, fry or boiled with piper nigrum and is considered a curative for anaemia and piles 21 .Though the consumption of the fish is limited to tribal people, it is commercially very important for its high export demand.From the Noakhali area in Bangladesh alone an average of 28,000kg of fish per month is collected for export 22 .Recently however the abundance of this species is declining due to a variety of reasons like habitat destruction, aquatic pollution, indiscriminate use of pesticide and heavy fishing pressure.The species is gradually becoming a vulnerable one and a management programme to conserve the species is gradually becoming unavoidable.If species conservation and commercial aspect does not provide reason enough for undertaking a management programme then nutritional capacity of the species if found considerable should certainly provide good reason and strong motivation.However, only a few works so far has been reported to determine the nutritional capacity of the fish species Monopterus 23 .We, in our laboratory have taken up the task of determining the nutritional capacity of Monopterus chuchia in terms of its fatty acid content and hereby wish to report our interesting initial finding.

MATERIAL AND METHODS
Six fish samples were caught from the same pond in Panduah, W.B., India in January 2012.Average weight of the fish is around (500 ± 7.25) gms.The fishes killed by hitting on their heads were brought to the laboratory and stored at -30 o C in a freezer until they were used 24 .After measuring total length and weight of the fishes, their proximate compositions were analyzed according the procedure laid down in AOAC 25 .The edible portions (the muscle tissues of the body) were separated from the head, skin, bone and viscera.The body muscles were removed from the bones and cut into small pieces.They were then blended in a sterilized mixer grinder for 30 seconds.5gms of the minced pieces were used for extraction of total lipids with a chloroform-methanol mixture in accordance to the method of Folch et.al. 26 After extraction the crude lipid was purified, dried and weighed by using methods of Folch et.al. 26.The extracted lipids were esterified with BF 3 -MeOH and recovered in heptanes 25 .The purified fatty acid methyl esters (FAME) mixture obtained was analyzed using a Shimadzu Gas Chromatograph (Model: GC-2010, Shimadzu, Japan) with a Flame ionization detector (FID) on a split injector.A SP-2560 capillary column (100 m long x 0.25 mm i.d) was used for FAME analysis.Oxygen free nitrogen was used as a carrier gas at a flow rate of 33.9 ml/minute.The initial oven temperature was 140 °C for 5 minutes which was slowly raised to 240 °C at a rate of 4 °C / min.and finally held at 240 °C for 20 minutes.The injector and detector temperature were finally set at 260 °C.Volume injected 1 µl; split ratio, 1:30.Peaks obtained were identified by comparison of their retention times with that of standard fatty acid methyl esters.The percentage compositions of the samples under investigation were computed from the G.C peak areas.The results obtained were placed in Table 1.
Erucic acid which is known to be an anti-nutritional factor which induces an increased incidence of myocardial lipidosis in animal 31 is also present in this fish though in very small amount.
Another important aspect which must be noted is the n-3 : n-6 ratio of the unsaturated fatty acids of this fish which is an useful indicator for comparing relative nutritional values of fish oil.A ratio within 1.1 to 1.5 is considered healthy for human diet 32 .The n-3 : n-6 ratio of this fish is around 1.48 which suggests that this fish can be a good nutritious diet.
According to the recommendation of British Nutrition Foundation 33 , a persons balanced and healthy diet must contain 0.2 gm of EPA + DHA daily or at least 1.5 gms of EPA + DHA on a weekly basis.Based on the above finding and recommendation, one can easily conclude that Monopterus chuchia is a potentially nutritious food with health benefits for the population of West Bengal, India.