ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Abstract

New Analytical HPLC Method Development and Validation for the Simultaneous Quantification of Paritaprevir Ombitasvir and Ritonavir in Spiked Human Plasma

Phani R. S. Ch1 , K. R. S. Prasad1 and Useni Reddy Mallu2

DOI : http://dx.doi.org/10.13005/ojc/330528


Abstract:

A rapid HPLC bio-analytical method has been developed and validated for the simultaneous quantification of Ombitasvir (OMBTSR), Ritonavir (RTNVR), Paritaprevir (PTVR) in human plasma. OMBTSR and PTVR are used to control Hepatitis-C infection. RTNVR is used in the treatment of HIV/AIDS. The method was developed with Column (Intersil ODSC18, 250 mm × 4.6 mm×5µ) at230 nm wave length and at flow rate of 1.0mL/min. The mobile phase consisted of 20% Acetonitrile, 20%Methanol, 60%1mM NH4H2PO4Buffer(PH 6.5 v/v). The retention times of RTNVR, OMBTSR, PTVRare 5.7 min, 7.8min and 12.8 min respectively. The method was developed and validated in terms of linearity, interday precision, intraday precision, accuracy, LOQ, LOD and stability study. The proposed method is useful in pharmacokinetic studies using HPLC or LC-MS.

Keywords:

Ombitasvir; Ritonavir; Paritaprevir; HPLC Method; Bio Analytical Method

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