ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
     FacebookTwitterLinkedinMendeley

Abstract

Evaluation of A New Stability Indicating Method for the Determination of Aceclofenac and Thiocolchicoside in Pharmaceutical Dosage form by RP-HPLC.

Santhoshi G Rajamahanti1, Annapurna. N1, Santosh T2, Durga B1 and Raziya SK1

DOI : http://dx.doi.org/10.13005/ojc/330334


Abstract:

A new RP- HPLC method was developed and validated for the estimation of aceclofenac and thiocolchicoside in their tablet dosage form. The chromatographic separation was carried out by using Agilent Zorbax column (150 × 4.6mm, 5µm). The mobile phase containing the combination of methanol and 0.1% ortho phosphoric acid of 75:25 (v/v) ratios.  In this method the flow rate used is 1 min/ml for the separation at the detection wavelength of 275 nm. The proposed method was validated according to the ICH guidelines. The method was linear (R2 > 0.99), precise (RSD < 2.0%), accurate, simple, sensitive and robust. This method can be successfully used for the routine pharmaceutical analysis.  

Keywords:

Thiocolchicoside; Aceclofenac; RP-HPLC; Method development; Validation

[ View HTML Full Text]

Back to TOC